Saito Yoshiro, Yamada Atsushi, Suzuki Dai, Tanaka Junichi, Nagahama Ryo, Kurosawa Tamaki, Maki Koutaro, Mishima Kenji, Shirota Tatsuo, Kamijo Ryutaro
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan.
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
Genom Data. 2015 May 30;5:115-9. doi: 10.1016/j.gdata.2015.05.012. eCollection 2015 Sep.
Aging, also called senescence, is thought to be a physiological phenomenon that commonly occurs in various organs and tissues (Enoki et al., 2007 [1]). Many older adults experience dysfunction in their salivary glands, for example xerostomia, which is defined as dry mouth resulting from reduced or absent saliva flow (Nagler et al., 2004 [2]). In the present study, we investigated gene expression in submandibular glands of young (8 weeks old) and adult (50 weeks old) mice to analyze association of aging with gene expression profiling in mouse submandibular glands. Whole-genome gene expression profiles were analyzed using an Illumina Sentrix system with Mouse-WG-6 v.2 Expression BeadChips (Illumina). Of the genes screened, 284 showed detection values at a significance level of P < 0.01. Among those, the expression of 94 genes (33%) showed a greater decrease in adult mice as compared to young mice. On the other hand, that of 190 genes (77%) was increased in the adults more than in young mice. The data obtained in this study are publicly available in the Gene Expression Omnibus (GEO) database (accession number GSE66857).
衰老，也称为老化，被认为是一种常见于各种器官和组织的生理现象（榎木等，2007 [1]）。许多老年人会出现唾液腺功能障碍，例如口干症，其定义为由于唾液分泌减少或缺乏导致的口腔干燥（纳格勒等，2004 [2]）。在本研究中，我们调查了年轻（8周龄）和成年（50周龄）小鼠下颌下腺中的基因表达，以分析衰老与小鼠下颌下腺基因表达谱的关联。使用Illumina Sentrix系统和Mouse-WG-6 v.2表达微珠芯片（Illumina）分析全基因组基因表达谱。在筛选出的基因中，284个基因的检测值在P < 0.01的显著水平。其中，与年轻小鼠相比，94个基因（33%）的表达在成年小鼠中下降幅度更大。另一方面，190个基因（77%）的表达在成年小鼠中比在年轻小鼠中增加得更多。本研究获得的数据可在基因表达综合数据库（GEO）（登录号GSE66857）中公开获取。
