Kumar Manu, Kim Seong Ryong, Sharma Prabodh Chander, Pareek Ashwani
Department of Life Science, Sogang University, Seoul 121-742, South Korea.
Crop Improvement Division, Central Soil Salinity Research Institute, Karnal 132 001 (Haryana) India.
Genom Data. 2015 Jun 11;5:218-22. doi: 10.1016/j.gdata.2015.06.006. eCollection 2015 Sep.
There are many ways to detect polymorphism. In this study we use the microsatellite markers to detect the polymorphism for the salt tolerance. This method has been successfully conducted in Oryza sativa and Brassica juncea. The results are reproducible. In contrast to previous methods, our method is simple and quite accurate for detecting the polymorphic bands. In this study instead of using agarose gel and ethidium bromide staining, we used non-denaturing polyacrylamide gel and a low-cost improved method for silver staining when we compare it to 11 other methods for their ability to detect simple sequence repeat polymorphisms as small as 50 bp in denaturing polyacrylamide gels. All methods detected the same alleles and banding pattern. However, important differences in sensitivity, contrast, time consumption and background were observed.
检测多态性有多种方法。在本研究中，我们使用微卫星标记来检测耐盐性的多态性。该方法已在水稻和芥菜中成功应用。结果具有可重复性。与先前的方法相比，我们的方法在检测多态性条带方面简单且相当准确。在本研究中，当我们将其与其他11种在变性聚丙烯酰胺凝胶中检测小至50 bp的简单序列重复多态性的方法进行比较时，我们使用非变性聚丙烯酰胺凝胶和一种低成本的改良银染方法，而不是使用琼脂糖凝胶和溴化乙锭染色。所有方法都检测到相同的等位基因和条带模式。然而，在灵敏度、对比度、耗时和背景方面观察到了重要差异。
