Lang Lina, Xu Aixia, Ding Juan, Zhang Yan, Zhao Na, Tian Zhengshu, Liu Yaping, Wang Yang, Liu Xia, Liang Fenghao, Zhang Bingbing, Qin Mengfan, Dalelhan Jazira, Huang Zhen
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, China.
Front Plant Sci. 2017 Jun 14;8:1000. doi: 10.3389/fpls.2017.01000. eCollection 2017.
Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in . Up to 45 quantitative trait loci (QTLs) for 10 indicators were identified in the F populations. These QTLs can account for 4.80-51.14% of the phenotypic variation. A major QTL, qSPAD5 on LG5 associated with chlorophyll can be detected in three replicates. Two intron polymorphic (IP) markers in this QTL region were developed successfully to narrow down the QTL location to a region of 390 kb. A salt tolerance related gene was primary identified as the candidate gene in this region. The full length of the candidate gene was 1,063 bp containing three exons and two introns in L. The open reading frame (ORF) is 867 bp and encodes 287 amino acids. Three amino acid differences (34, 54, and 83) in the conserved domain (B-box) were identified. RT-qPCR analysis showed that the gene expression had significant difference between the two parents. The study laid great foundation for salt tolerance related gene mapping and cloning in L.
盐胁迫是严重限制作物产量的典型非生物胁迫之一。在本研究中，构建了一张基于532个分子标记、覆盖1341.1厘摩的遗传连锁图谱，以鉴定与[作物名称]耐盐性相关的位点。在F群体中，针对10个指标共鉴定出45个数量性状位点（QTL）。这些QTL可解释表型变异的4.80 - 51.14%。一个与叶绿素相关的主效QTL，LG5上的qSPAD5，在三次重复中均能检测到。在该QTL区域成功开发了两个内含子多态性（IP）标记，将QTL定位范围缩小至390 kb的区域。在该区域初步鉴定出一个与耐盐性相关的基因作为候选基因。候选基因全长1063 bp，在[作物名称]中包含三个外显子和两个内含子。开放阅读框（ORF）为867 bp，编码287个氨基酸。在保守结构域（B-box）中鉴定出三个氨基酸差异（34、54和83）。RT-qPCR分析表明，该基因在两个亲本间的表达存在显著差异。该研究为[作物名称]耐盐相关基因的定位和克隆奠定了重要基础。
