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使用椭球面镜提高层析衍射显微镜的纵向分辨率。

Improved longitudinal resolution in tomographic diffractive microscopy with an ellipsoidal mirror.

作者信息

Ding C, Tan Z

机构信息

College of Sciences, Huazhong Agricultural University, Wuhan, China.

Institute of Applied Physics, Huazhong Agricultural University, Wuhan, China.

出版信息

J Microsc. 2016 Apr;262(1):33-9. doi: 10.1111/jmi.12338. Epub 2015 Oct 20.

Abstract

Tomographic diffractive microscopy is a technique, which is able to image transparent unstained samples with high resolution. The three-dimensional distribution of the complex refractive index can be reconstructed quantitatively from the measured scattered fields under various illumination and detection angles, according to the diffraction tomography theorem. We propose a tomographic diffractive microscopy setup with an ellipsoidal mirror as the light collector. We demonstrate analytically and with numerical simulation that this approach permits to obtain images with drastically improved resolution.

摘要

层析衍射显微镜是一种能够对透明未染色样本进行高分辨率成像的技术。根据衍射层析定理,在不同的照明和检测角度下,通过测量散射场可以定量重建复折射率的三维分布。我们提出了一种以椭球面镜作为光收集器的层析衍射显微镜装置。我们通过解析和数值模拟证明,这种方法能够获得分辨率大幅提高的图像。

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