Laboratoire MIPS-EA2332, Université de Haute-Alsace, IUT Mulhouse, Mulhouse, France.
J Biophotonics. 2010 Jul;3(7):462-7. doi: 10.1002/jbio.200900094.
The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique.
作者开发了一种层析衍射显微镜，它将微全息术与角合成孔径照明相结合。它可以根据样品的复折射率分布（折射率和吸收）对其进行成像，并允许对未标记的样品进行高横向分辨率成像。作者现在研究将其用于生物应用，并使用荧光共焦显微镜和层析衍射显微镜对几种制剂进行了成像。结果突出了该仪器的一些有趣特性，这应该会引起生物学家对这项新技术的兴趣。
