免疫层析法和酶联免疫吸附测定法检测腹泻样本中贾第鞭毛虫粪便抗原

DETECTION OF GIARDIA INTESTINALIS COPROANTIGENS IN DIARRHEIC SAMPLES BY IMMUNOCHROMATOGRAPHIC AND ELISATECHNIQUES.

作者信息

Selim Monira Abd El-Wahab, Taha Afaf Abd El-Raouf, El-Aal Naglaa Fathy Abd, Farag Tahani Ismail, Yousef Asmaa Mohammed

出版信息

J Egypt Soc Parasitol. 2015 Aug;45(2):273-83. doi: 10.12816/0017571.

Abstract

Giardia intestinalis is one of the most common diarrhea-causing protozoa. The present study aimed to search for specific and sensitive diagnostic tests to avoid loss of infected cases with Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through comparison between direct parasitological method, an enzyme linked immunosorbent assay (ELISA) and immunochromatographic test (ICT). A comparative cross-sectional study including 75 cases suffering from diarrhea and other gastrointestinal symptoms suggestive of intestinal giardiasis as abdominal distention, abdominal pain, anorexia, nausea, vomiting and weight loss, and 25 cases were without any clinical manifestations enrolled in this study. For every case, complete history taking and full clinical examination were done. Stool samples were collected from all cases and investigated by direct parasitological method, ELISA, and immunochromatographic techniques. The results showed that the sensitivity of immunochromatographic technique was 96% and specificity was 96% while sensitivity of ELISA was 98% and specificity was 96% on comparing their results to the microscopic examination of stool samples for Giardia intestinalis.

摘要

肠道贾第虫是最常见的致腹泻原生动物之一。本研究旨在寻找特异性和敏感性高的诊断试验，通过比较直接寄生虫学方法、酶联免疫吸附测定（ELISA）和免疫层析试验（ICT），检测腹泻样本中的肠道贾第虫粪抗原，以避免肠道贾第虫感染病例的漏诊。一项比较性横断面研究纳入了75例患有腹泻及其他提示肠道贾第虫病的胃肠道症状（如腹胀、腹痛、厌食、恶心、呕吐和体重减轻）的患者，以及25例无任何临床表现的患者。对每例患者均进行了完整的病史采集和全面的临床检查。从所有患者中采集粪便样本，并采用直接寄生虫学方法、ELISA和免疫层析技术进行检测。将ELISA和免疫层析试验的结果与粪便样本中肠道贾第虫的显微镜检查结果进行比较，结果显示免疫层析试验的敏感性为96%，特异性为96%，而ELISA的敏感性为98%，特异性为96%。