Shaban Aly Nagwa, Bayoumi Ibrahim, Selem Rabab, Kardoush Manal, Rashed Gehan, Moharam Ahlam
Dept. of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt.
Dept. of Immunology and Evaluation of Drug, Theodor Bilharz Research Institute, Ciza, Egypt.
Iran J Parasitol. 2018 Oct-Dec;13(4):532-540.
The traditional basis of diagnosis is identification of trophozoites or cysts in the stool of infected patients. Recently the advent of more objective techniques like antigen detection methods has led to an increase in their use versus those that rely on subjective microscopic examination of fecal specimens for cysts may facilitate diagnosis of in stool specimens.
This cross-sectional study was carried out from Oct 2015 to Feb 2016 on patients admitted to Benha University Hospitals (Benha, Egypt) and outpatients of Theodor Bilharz Research Institute (TBRI) (Giza, Egypt). Purified cysts antigen was prepared by two-phase sucrose gradient technique. Polyclonal antibody against purified cysts antigen was prepared and labeled with horseradish peroxidase and Nano Magnetic Beads (NMB) to be used as detecting antibody. A total of 72 stool samples, 32 samples positive for giardiasis, 20 samples positive for other parasitic infections in addition to 20 negative samples were examined using dot ELISA and NMB dot-ELISA.
The sensitivity of the traditional dot-ELISA was 81.3 % and it increased by using the NMB-dot-ELISA to be 96.9% in stool samples. Specificity of both techniques was 97.5%.
Diagnosis of by NMB-Dot-ELISA technique is sensitive, specific, rapid and easy to perform and interpret. In this study, using the nano-magnetic beads increased the sensitivity of the applied technique.
传统的诊断依据是在感染患者的粪便中识别滋养体或包囊。最近，抗原检测方法等更客观技术的出现导致其使用量相对于那些依靠对粪便标本进行主观显微镜检查来检测包囊的方法有所增加，这可能有助于粪便标本中[疾病名称]的诊断。
这项横断面研究于2015年10月至2016年2月在贝纳大学医院（埃及贝纳）收治的患者以及西奥多·比尔哈兹研究所（埃及吉萨）的门诊患者中进行。通过两相蔗糖梯度技术制备纯化的[疾病名称]包囊抗原。制备针对纯化的[疾病名称]包囊抗原的多克隆抗体，并用辣根过氧化物酶和纳米磁珠（NMB）进行标记，用作检测抗体。使用斑点酶联免疫吸附测定法（dot ELISA）和NMB斑点酶联免疫吸附测定法对总共72份粪便样本进行检测，其中32份贾第虫病阳性样本，20份除贾第虫病外其他寄生虫感染阳性样本以及20份阴性样本。
传统的dot ELISA的灵敏度为81.3%，在粪便样本中使用NMB - dot ELISA后灵敏度提高到96.9%。两种技术的特异性均为97.5%。
NMB - Dot - ELISA技术对[疾病名称]的诊断灵敏、特异、快速且易于操作和解读。在本研究中，使用纳米磁珠提高了所应用技术的灵敏度。
