粪便抗原检测与直接检测方法对国家肝脏研究所患者蓝氏贾第鞭毛虫病的诊断比较
Copro-antigen detection versus direct methods for the diagnosis of Giardia lamblia in patients from the National Liver Institute.
作者信息
Selim Sahar, Nassef Nashaat, Sharaf Soraya, Badra Gamal, Abdel-Atty Dalia
机构信息
Department of Parasitology, National Liver Institute, Egypt.
出版信息
J Egypt Soc Parasitol. 2009 Aug;39(2):575-83.
In this study a commercially available immunoenzymatic assay to detect G. lamblia specific copro-antigen was evaluated. A total of 90 stool samples were tested. Diagnosis of giardiasis by ELISA for copro-Ag detection was positive in 46 (51.1%) patients whereas by direct stool analysis after formol ether concentration G. lamblia was detected in 38 (42.2%) patients only. ELISA technique for detection of Giardia copro-antigen had a sensitivity of 97.3% and a specificity of 82.6% with PPV of 80.4% and a NPV of 97.7%.
在本研究中，对一种用于检测蓝氏贾第鞭毛虫特异性粪便抗原的市售免疫酶测定法进行了评估。共检测了90份粪便样本。通过酶联免疫吸附测定法（ELISA）检测粪便抗原诊断贾第虫病，46例（51.1%）患者呈阳性，而经甲醛乙醚浓缩后直接粪便分析仅在38例（42.2%）患者中检测到蓝氏贾第鞭毛虫。检测贾第虫粪便抗原的ELISA技术敏感性为97.3%，特异性为82.6%，阳性预测值为80.4%，阴性预测值为97.7%。
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