Wang Aishuai, Sun Yuena, Wu Changwen
a Laboratory of Fish Biogenetics & Immune Evolution , College of Marine Science, Zhejiang Ocean University , Zhoushan , China and.
b National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University , Zhoushan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4444-4446. doi: 10.3109/19401736.2015.1089563. Epub 2015 Oct 21.
The complete mitochondrial genome of the Cheilodactylus quadricornis was firstly determined in the present study. The mitochondrial genome of C. quadricornis is 16 521 nucleotides, comprising 13 protein-coding genes and 2 ribosomal RNA genes, 22 tRNA genes and 2 main non-coding regions (the control region and the origin of the light-strand replication). The overall base composition was T, 26.3%; C, 29.6%; A, 27.8% and G, 16.3%. The gene arrangement, base composition, and tRNA structures of the complete mitochondrial genome of C. quadricornis is similar to other teleosts. Only two central conserved sequence blocks (CSB-2 and CSB-3) were identified in the control region. In addition, the conserved motif 5'-GCCGG-3' was identified in the origin of light-strand replication of C. quadricornis. The complete mitochondrial genome of C. quadricornis was used to construct phylogenetic tree, which shows that C. quadricornis and C. variegatus clustered in a clade and formed a sister relationship. This mitogenome sequence data would play an important role in population genetics and phylogenetic analysis of the Cheilodactylidae.
本研究首次测定了四棘唇指䱵的完整线粒体基因组。四棘唇指䱵的线粒体基因组为16521个核苷酸，包括13个蛋白质编码基因、2个核糖体RNA基因、22个tRNA基因和2个主要的非编码区（控制区和轻链复制起点）。总体碱基组成为：T占26.3%、C占29.6%、A占27.8%、G占16.3%。四棘唇指䱵完整线粒体基因组的基因排列、碱基组成和tRNA结构与其他硬骨鱼相似。在控制区仅鉴定出两个中央保守序列块（CSB-2和CSB-3）。此外，在四棘唇指䱵的轻链复制起点鉴定出保守基序5'-GCCGG-3'。利用四棘唇指䱵的完整线粒体基因组构建系统发育树，结果表明四棘唇指䱵和多斑唇指䱵聚在一个分支中，形成姐妹关系。该线粒体基因组序列数据将在唇指䱵科的群体遗传学和系统发育分析中发挥重要作用。
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