Wang Zhongquan, Qin Kaili, Liu Jingxi, Song Na, Han Zhiqiang, Gao Tianxiang
a Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Marine Ecology , Yantai , People's Republic of China.
b Institute of Evolution and Marine Biodiversity, Ocean University of China , Qingdao , People's Republic of China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4447-4448. doi: 10.3109/19401736.2015.1089564. Epub 2016 Mar 17.
In this study, the complete mitochondrial genome (mitogenome) sequence of Pholis nebulosus has been determined by long polymerase chain reaction and primer-walking methods. The mitogenome is a circular molecule of 16 524 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region), the gene contents of which are identical to those observed in most bony fishes. Within the control region, we identified the termination-associated sequence domain (TAS), and the conserved sequence block domain (CSB-F, CSB-E, CSB-D, CSB-C, CSB-B, CSB-A, CSB-1, CSB-2, CSB-3).
在本研究中,通过长聚合酶链反应和引物步移法测定了云斑鳞鱼的完整线粒体基因组(线粒体基因组)序列。该线粒体基因组是一个长度为16524 bp的环状分子,包括13个蛋白质编码基因、2个核糖体RNA基因、22个转移RNA基因和2个非编码区(轻链复制起点和控制区)的典型结构,其基因组成与大多数硬骨鱼中观察到的相同。在控制区内,我们鉴定出了终止相关序列结构域(TAS)以及保守序列框结构域(CSB-F、CSB-E、CSB-D、CSB-C、CSB-B、CSB-A、CSB-1、CSB-2、CSB-3)。