Wu Renxie, Niu Sufang, Qin Chuanxin, Liu Jing
a College of Fisheries, Guangdong Ocean University , Zhanjiang , Guangdong , PR China.
b South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , Guangdong , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4624-4626. doi: 10.3109/19401736.2015.1101587. Epub 2015 Nov 5.
The complete mitogenome of the yellowbelly threadfin bream, Nemipterus bathybius, has been reported for the first time in this study. The circular mtDNA molecule was 17 353 bp in size and the overall nucleotide composition of H-strand was 29.0% A, 28.3% T, 16.5% G, and 26.2% C, with an A + T bias. The complete mitogenome encoded 13 protein-coding genes, 2 rRNAs, 22 tRNAs, and 2 non-coding regions, as found in other typical vertebrate mitogenomes. The D-loop located between tRNA and tRNA genes and the length was 1603 bp, rich in A + T (73.0%). The phylogenetic tree of complete mitogenome sequences of 12 families and 26 species showed that the family Nemipteridae was in the basal position of the suborder Percoidei, whereas its two associated families (Sparidae and Lethrinidae) were clustered together with other 7 families, which supported a non-monophyly of superfamily Sparoidea.
本研究首次报道了黄斑金线鱼(Nemipterus bathybius)的完整线粒体基因组。环状线粒体DNA分子大小为17353 bp,H链的总体核苷酸组成为29.0%A、28.3%T、16.5%G和26.2%C,具有A+T偏向性。完整的线粒体基因组编码13个蛋白质编码基因、2个rRNA、22个tRNA和2个非编码区,这与其他典型脊椎动物线粒体基因组的情况一致。D环位于tRNA基因之间,长度为1603 bp,富含A+T(73.0%)。12科26种鱼类完整线粒体基因组序列的系统发育树表明,金线鱼科处于鲈亚目基部位置,而与其相关的两个科(鲷科和裸颊鲷科)与其他7个科聚在一起,这支持了鲷形总科的非单系性。