Biller T, Yosipovitch Z, Gedalia I
J Dent Res. 1977 Jan;56(1):53-6. doi: 10.1177/00220345770560011201.
The effect of the local application of fluoride on the healing process of experimental calvarial defects in rats was studied. A total of 172 male rats, 6 weeks old, weighing 100 to to 160 gm, were used in the experiments. Two standard defects, each approximately 2.8 mm in diameter, were drilled through the parietal bones on either side of the sagittal suture. In one half of the rats that were operated on, a 20-minute topical application of 2% acidulated (0.1 M H3PO4) NaF solution was used. The control rats underwent the same local treatment with saline solution. Groups of 9 to 13 rats were killed at 4, 5, 7, 9, and 12 weeks after the operation. Radiographs of each calvarium demonstrated the healing area in the bone. Linear bone apposition was measured from histological sections. Fluoride content was determined in ashed calvaria with an Orion electrode. The results indicate that all fluoride-treated groups had increased bone gain compared with the control group. The histological examination revealed no major differences in the structure, number, and size of the cells of the newly formed bone in either group. The mean fluoride concecreased until completion of the experiment.
研究了局部应用氟化物对大鼠实验性颅骨缺损愈合过程的影响。实验共使用了172只6周龄、体重100至160克的雄性大鼠。在矢状缝两侧的顶骨上钻出两个标准缺损，每个直径约2.8毫米。在一半接受手术的大鼠中，局部应用2%酸化（0.1M H3PO4）NaF溶液20分钟。对照大鼠用生理盐水进行相同的局部处理。术后4、5、7、9和12周处死9至13只大鼠组成的组。每个颅骨的X线片显示了骨中的愈合区域。从组织学切片测量线性骨沉积。用奥立龙电极测定灰化颅骨中的氟含量。结果表明，与对照组相比，所有氟处理组的骨生长均增加。组织学检查显示，两组新形成骨的细胞结构、数量和大小均无重大差异。实验结束前，平均氟含量降低。
