Ezirganlı Şeref, Kazancıoğlu Hakkı O, Mihmanlı Ahmet, Aydın Mehmet Ş, Sharifov Rasul, Alkan Alpay
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, İstanbul, Turkey.
Clin Oral Implants Res. 2014 Aug;25(8):969-76. doi: 10.1111/clr.12177. Epub 2013 Apr 21.
To evaluate bone-healing effects of local simvastatin application to critical size defects (CSDs) in the experimental diabetes mellitus (DM) rat model.
A total of 35 male Sprague-Dawley rats with an average weight of 350 g and aged 3 months were used in this study. The rats were divided into five groups of seven animals each: passive control (group A), active control (group B), 0.5 mg simvastatin (group C), 1.0 mg simvastatin (group D), and 1.5 mg simvastatin (group E). Streptozotocin was used to induce Type 1 diabetes in all rats. Eight mm CSDs were created under anesthesia in each rat calvarium. CSDs were left empty in group A. Defects in group B were grafted alone with a gelatin sponge mixed with normal saline. Defects in the experimental groups (groups A, B, and C) were grafted with gelatin sponge mixed saline solutions contain 0.5, 1.0, 1.5 mg simvastatin. Rats were sacrificed after 1 month, and the defects were prepared for radiologic and histomorphometric assessment of regenerated bone.
None of the specimens exhibited complete closure of new bone across the 8-mm defect. A correlation between computed tomography and histomorphometric analysis was not determined. Both amount of volume and area of regenerated bone were found higher in the experimental groups than in the control groups. However, these values were not found statistically significant degree (P < 0.05) for each groups. The density of regenerated bone in the region of interest was higher in the control groups in contrast to in the experimental groups. However, statistical significance was just found between groups C and A and between groups C and B (P < 0.05).
The local simvastatin application enhanced healing of the bone defects in the diabetic rat model CSDs.
评估在实验性糖尿病（DM）大鼠模型中，局部应用辛伐他汀对临界尺寸骨缺损（CSDs）的骨愈合效果。
本研究共使用35只平均体重350克、3月龄的雄性Sprague-Dawley大鼠。将大鼠分为五组，每组7只：被动对照组（A组）、主动对照组（B组）、0.5毫克辛伐他汀组（C组）、1.0毫克辛伐他汀组（D组）和1.5毫克辛伐他汀组（E组）。所有大鼠均使用链脲佐菌素诱导1型糖尿病。在麻醉下于每只大鼠颅骨上制造8毫米的CSDs。A组的CSDs保持为空。B组的缺损单独用与生理盐水混合的明胶海绵移植。实验组（C、D、E组）的缺损用含有0.5、1.0、1.5毫克辛伐他汀的明胶海绵混合盐溶液移植。1个月后处死大鼠，对缺损部位进行再生骨的放射学和组织形态计量学评估。
所有标本均未显示8毫米缺损处新骨完全闭合。未确定计算机断层扫描与组织形态计量学分析之间的相关性。发现实验组再生骨的体积和面积均高于对照组。然而，这些值在各组间未发现具有统计学显著性差异（P < 0.05）。与实验组相比，对照组感兴趣区域再生骨的密度更高。然而，仅在C组与A组之间以及C组与B组之间发现有统计学显著性差异（P < 0.05）。
局部应用辛伐他汀可促进糖尿病大鼠模型CSDs的骨缺损愈合。
