Koza V, Cetkovský P
Vnitr Lek. 1989 Jan;35(1):16-22.
The authors describe the indirect immunoperoxidase method which makes possible immunophenotyping of cells on dried smears of peripheral and bone marrow. They used only local currently available monoclonal antibodies with a diagnostic value in lymphoproliferative conditions. The above procedure makes it possible to examine stored non-fixed smears, to evaluate the morphology of positive and negative cells and to evaluate findings retrospectively.
作者描述了间接免疫过氧化物酶法,该方法能够对外周血和骨髓涂片上的细胞进行免疫分型。他们仅使用了目前当地可获得的、在淋巴增殖性疾病中有诊断价值的单克隆抗体。上述方法能够对储存的未固定涂片进行检查,评估阳性和阴性细胞的形态,并对结果进行回顾性评估。