Erber W N, Mynheer L C, Mason D Y
Lancet. 1986 Apr 5;1(8484):761-5. doi: 10.1016/s0140-6736(86)91781-2.
The reliability of labelling routinely prepared air-dried peripheral-blood and bone-marrow smears with the alkaline-phosphatase/anti-alkaline-phosphatase (APAAP) method for phenotyping leukaemias was evaluated in 259 cases of haematological malignancy. All the cellular markers currently used in leukaemia diagnosis could be detected with this technique. Since the technique can be used on blood and marrow smears even after storage or postal transport, it is suggested that APAAP labelling should become the method of choice for immunophenotyping neoplastic samples in many haematology laboratories not equipped with specialised immunological diagnostic facilities. The method should also be valuable for epidemiological studies of leukaemia.
采用碱性磷酸酶/抗碱性磷酸酶（APAAP）法对白血病进行表型分析时，对常规制备的空气干燥外周血和骨髓涂片标记的可靠性，在259例血液系统恶性肿瘤病例中进行了评估。目前白血病诊断中使用的所有细胞标志物均可通过该技术检测到。由于该技术即使在储存或邮寄运输后仍可用于血液和骨髓涂片，因此建议在许多未配备专门免疫诊断设施的血液学实验室中，APAAP标记应成为肿瘤样本免疫表型分析的首选方法。该方法对白血病的流行病学研究也应具有重要价值。
