Wang Ning, Liang Bin, Huo Juan, Liang Wei
a Ministry of Education Key Laboratory for Tropical Plant and Animal Ecology, College of Life Sciences, Hainan Normal University , Haikou , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4409-4410. doi: 10.3109/19401736.2015.1089547. Epub 2015 Oct 21.
The complete mitochondrial genome of the Lesser Cuckoo Cuculus poliocephalus was determined using next-generation sequencing. Phylogenetic analyses with 20 cuckoo species and six genes (12S, ATP6, CYTb, ND2, ND3, and ND6) strongly verified the identity of our sample as grouping with C. poliocephalus, which was sister to a clade of other Cuculus spp., including C. canorus, C. micropterus, and C. saturatus. The mitogenomic length of C. poliocephalus was 17 508 bp, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, one control region with 48 bp unit tandem repeats close to 3' end, and one short pseudo-control region with 33 tandem repeats of CAACAAA. An extra nucleotide (T) was identified at position 174 of ND3. The mitogenome of C. poliocephalus will contribute to studies of mitogenomic evolution, the phylogenetic relationship of cuckoos, and the co-evolutionary pattern between brood parasitic birds and their hosts.
利用下一代测序技术测定了小杜鹃（Cuculus poliocephalus）的完整线粒体基因组。对20种杜鹃物种和六个基因（12S、ATP6、CYTb、ND2、ND3和ND6）进行的系统发育分析有力地证实了我们样本与小杜鹃的一致性，小杜鹃与包括大杜鹃（C. canorus）、中杜鹃（C. micropterus）和四声杜鹃（C. saturatus）在内的其他杜鹃属物种的一个分支构成姐妹关系。小杜鹃的线粒体基因组长度为17508 bp，包括13个蛋白质编码基因、22个转运RNA基因、两个核糖体RNA基因、一个靠近3'端具有48 bp单位串联重复的控制区，以及一个具有33个CAACAAA串联重复的短假控制区。在ND3的第174位鉴定出一个额外的核苷酸（T）。小杜鹃的线粒体基因组将有助于线粒体基因组进化、杜鹃系统发育关系以及巢寄生鸟类与其宿主之间协同进化模式的研究。
