Taruya T, Takeno S, Kubota K, Sasaki A, Ishino T, Hirakawa K
Department of Otolaryngology, Head and Neck Surgery,Division of Clinical Medical Science,Programs for Applied Biomedicine,Graduate School of Biomedical Sciences,Hiroshima University,Hiroshima,Japan.
J Laryngol Otol. 2015 Dec;129(12):1194-200. doi: 10.1017/S0022215115002728. Epub 2015 Oct 21.
Although human paranasal sinuses are critical organs for nitric oxide production, little information is available regarding the role of arginase in alterations of arginine metabolism and nasal nitric oxide levels that may be informative for classifying chronic rhinosinusitis subtypes.
The expression and localisation of arginase and nitric oxide synthase isoforms in paranasal sinus mucosa were examined, and the fractional exhaled nitric oxide was measured in chronic rhinosinusitis without nasal polyps (n=18) and chronic rhinosinusitis with nasal polyps (n = 27) patients.
Increased arginase-2 activities in chronic rhinosinusitis without nasal polyps patients were associated with significantly lower levels of nasal fractional exhaled nitric oxide. Chronic rhinosinusitis with nasal polyps patients showed significant NOS2 messenger RNA upregulation with concomitant higher levels of oral and nasal fractional exhaled nitric oxide.
These results indicate that fractional exhaled nitric oxide is a valid marker for differentiating chronic rhinosinusitis phenotypes based on a delicate balance between arginase and nitric oxide synthase activities in nitric oxide production.
尽管人类鼻窦是产生一氧化氮的关键器官，但关于精氨酸酶在精氨酸代谢改变及鼻一氧化氮水平变化中的作用，目前所知甚少，而这些信息可能有助于对慢性鼻窦炎亚型进行分类。
检测鼻窦黏膜中精氨酸酶和一氧化氮合酶亚型的表达及定位，并对无鼻息肉的慢性鼻窦炎患者（n = 18）和有鼻息肉的慢性鼻窦炎患者（n = 27）测量呼出一氧化氮分数。
无鼻息肉的慢性鼻窦炎患者中精氨酸酶-2活性增加与呼出一氧化氮分数显著降低相关。有鼻息肉的慢性鼻窦炎患者显示出显著的NOS2信使核糖核酸上调，同时伴有口腔和呼出一氧化氮分数升高。
这些结果表明，呼出一氧化氮分数是基于一氧化氮产生过程中精氨酸酶和一氧化氮合酶活性之间的微妙平衡来区分慢性鼻窦炎表型的有效标志物。
