Vlad Diana, Albu Silviu
Second Department of Otolaryngology, University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400489, Romania.
Department of ENT, University of Medicine and Pharmacy Iuliu Hatieganu from Cluj-Napoca, Cluj Napoca 400006, Romania.
J Clin Med. 2019 Nov 1;8(11):1809. doi: 10.3390/jcm8111809.
Nitric oxide (NO) has emerged as an important regulator of upper airway inflammation, mainly as part of the local naso-sinusal defense mechanisms. Increased arginase activity can reduce NO levels by decreasing the availability of its precursor, L-arginine. Chronic rhinosinusitis (CRS) has been associated with low levels of nasal nitric oxide (nNO). Thus, the present study investigates the activity of arginase I (ARG1) and II (ARG2) in CRS and its possible involvement in the pathogenesis of this disease. Under endoscopic view, tissue samples of pathologic ( = 36) and normal ( = 29) rhinosinusal mucosa were collected. Arginase I and II mRNA levels were measured using real-time PCR. Our results showed low arginase I activity in all samples. The levels of ARG2 were significantly higher in patients with chronic rhinosinusitis compared to the control group (fold regulation (FR) 2.22 ± 0.42 vs. 1.31 ± 0.21, = 0.016). Increased ARG2 expression was found in patients with CRS without nasal polyposis (FR 3.14 ± 1.16 vs. 1.31 ± 0.21, = 0.0175), in non-allergic CRS (FR 2.55 ± 0.52 vs. 1.31 ± 0.21, = 0.005), and non-asthmatic CRS (FR 2.42 ± 0.57 vs. 1.31 ± 0.21, = 0.028). These findings suggest that the upregulation of ARG2 may play a role in the pathology of a distinctive phenotype of CRS.
一氧化氮（NO）已成为上呼吸道炎症的重要调节因子，主要作为局部鼻窦防御机制的一部分。精氨酸酶活性增加可通过降低其前体L-精氨酸的可用性来降低NO水平。慢性鼻窦炎（CRS）与鼻一氧化氮（nNO）水平低有关。因此，本研究调查了CRS中精氨酸酶I（ARG1）和II（ARG2）的活性及其可能参与该疾病发病机制的情况。在内镜观察下，收集了病理（n = 36）和正常（n = 29）鼻窦黏膜的组织样本。使用实时PCR测量精氨酸酶I和II的mRNA水平。我们的结果显示所有样本中精氨酸酶I活性较低。与对照组相比，慢性鼻窦炎患者中ARG2的水平显著更高（倍数调节（FR）2.22±0.42对1.31±0.21，P = 0.016）。在无鼻息肉的CRS患者（FR 3.14±1.16对1.31±0.21，P = 0.0175）、非过敏性CRS（FR 2.55±0.52对1.31±0.21，P = 0.005）和非哮喘性CRS（FR 2.42±0.57对1.31±0.21，P = 0.028）中发现ARG2表达增加。这些发现表明，ARG2的上调可能在CRS独特表型的病理过程中起作用。
