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中国矮岩羊(Pseudois schaeferi haltenorth)的线粒体基因组全序列

The complete mitochondrial genome sequence of the dwarf blue sheep, Pseudois schaeferi haltenorth in China.

作者信息

Liu J B, Zeng Y F, Yuan C, Yue Y J, Ding X Z, Guo T T, Han J L, Feng R L, Sun X P, Niu C E, Yang B H, Guo J

机构信息

a Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences , Lanzhou , China and.

b Sheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences , Lanzhou , China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4411-4413. doi: 10.3109/19401736.2015.1089548. Epub 2015 Oct 21.

DOI:10.3109/19401736.2015.1089548
PMID:26488305
Abstract

The dwarf blue sheep (Pseudois schaeferi haltenorth) belongs the subfamily Caprinae, which is distributed in Sichuan, Tibet, Yunnan, and Qinghai in China. In this study, the complete mitochondrial genome of Pseudois schaeferi haltenorth was sequenced. The mitogenome was 16 741 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a non-coding control region (D-loop region). As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall base composition of the Pseudois schaeferi haltenorth is 33.54% A, 26.37% T, 26.91% C, and 13.18% G, A + T (59.91%) was higher than G + C (40.09%). The phylogenetic relationships was analyzed using the complete mitogenome sequence, results show that P. schaeferi haltenorth should be a different species differ from the Genus pseudois hodgson. These information provide useful data for further study on the protection of genetic resources and the taxonomy of Caprinae.

摘要

矮岩羊(Pseudois schaeferi haltenorth)属于羊亚科,分布于中国的四川、西藏、云南和青海。在本研究中,对矮岩羊的完整线粒体基因组进行了测序。该线粒体基因组长度为16741bp,由13个蛋白质编码基因、22个转运RNA(tRNA)基因、2个核糖体RNA(rRNA)基因和一个非编码控制区(D-loop区)组成。与其他哺乳动物一样,大多数线粒体基因由重链编码,除了ND6和8个tRNA基因由轻链编码。矮岩羊的总体碱基组成为A占33.54%、T占26.37%、C占26.91%、G占13.18%,A+T(59.91%)高于G+C(40.09%)。利用完整的线粒体基因组序列分析了系统发育关系,结果表明矮岩羊应是一个与贺氏岩羊属不同的物种。这些信息为进一步研究遗传资源保护和羊亚科分类学提供了有用的数据。

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