Terouanne B, Alameddine S, Martin J L, Nicolas J C, Cristol P, Sultan C, Crastes de Paulet A
INSERM U58 60 Montpellier.
Ann Biol Clin (Paris). 1989;47(1):15-21.
The authors describe a bioluminescent immunoassay of LH in plasma and urine. It uses two monoclonal antibodies, one is labelled with glucose-6-phosphate-dehydrogenase, the other one is coimmobilized together with bioluminescent enzymes from marine bacteria on the same adsorbent (Sepharose). This assay can be performed directly on 20 microliters plasma or 10 microliters urine. The protocol is very fast, no separation step is required to remove the excess labeled antibodies. The inhibitory effect of the biological sample on luminescent reaction is determined by adding NADH to the assay tubes. The working range of this assay is 3 to 300 Ul/l, with a sensitivity of detection of 0.5 Ul/l. Recovery, linearity, within and between assay precision were evaluated and appeared to be satisfactory. The authors have observed a good correlation between results obtained with our method and with radioimmunoassay.
作者描述了一种用于检测血浆和尿液中促黄体生成素（LH）的生物发光免疫测定法。该方法使用两种单克隆抗体，一种用葡萄糖-6-磷酸脱氢酶标记，另一种与来自海洋细菌的生物发光酶共同固定在同一吸附剂（琼脂糖）上。此测定法可直接对20微升血浆或10微升尿液进行检测。该检测方案非常快速，无需分离步骤来去除过量的标记抗体。通过向测定管中添加烟酰胺腺嘌呤二核苷酸（NADH）来确定生物样品对发光反应的抑制作用。该测定法的工作范围为3至300国际单位/升，检测灵敏度为0.5国际单位/升。对回收率、线性、批内和批间精密度进行了评估，结果似乎令人满意。作者观察到用我们的方法获得的结果与放射免疫测定法之间具有良好的相关性。
