甲胎蛋白的生物发光免疫测定法。

Bioluminescent immunoassay for alpha-fetoprotein.

作者信息

Térouanne B, Nicolas J C, Crastes de Paulet A

出版信息

Anal Biochem. 1986 Apr;154(1):132-7. doi: 10.1016/0003-2697(86)90506-3.

Abstract

A bioluminescent immunoassay of alpha-fetoprotein is described. It uses monoclonal antibodies labeled with glucose-6-phosphate dehydrogenase and polyclonal antibodies coimmobilized on Sepharose with bioluminescent enzymes from marine bacteria. The bioluminescent reaction which occurs in the immunosorbent is proportional to the amount of alpha-fetoprotein in the assay. The protocol is simple and rapid, and no separation step is required to remove the excess labeled antibodies. The assay can be performed directly on 25 microliters serum and it is as sensitive as other immunometric assays.

摘要

本文描述了一种甲胎蛋白的生物发光免疫测定法。它使用用葡萄糖 -6-磷酸脱氢酶标记的单克隆抗体和与来自海洋细菌的生物发光酶共同固定在琼脂糖上的多克隆抗体。在免疫吸附剂中发生的生物发光反应与测定中甲胎蛋白的量成正比。该方法简单快速，无需分离步骤去除过量的标记抗体。该测定法可直接在25微升血清上进行，并且与其他免疫测定法一样灵敏。