Frosinski J, Hermann B, Maier K, Boden K
Centre for Infectious Diseases and Infection Control,University Hospital Jena,Germany.
Institute of Medical Microbiology,University Hospital Jena,Germany.
Epidemiol Infect. 2016 Apr;144(6):1148-52. doi: 10.1017/S0950268815002447. Epub 2015 Oct 22.
Large outbreaks of Q fever have recently increased the awareness of this disease as a public health issue. Knowledge of the general impact of Q fever relies mainly on seroprevalence studies and it is fundamental that seroprevalence is assessed accurately. Therefore we evaluated the few enzyme-linked immunosorbent assays (ELISAs) commercially available for this purpose. An outbreak in 2005 in Jena, a city of 100,000 inhabitants, gave us the opportunity for the evaluation. However, we found disappointingly low sensitivities for two (42% and 51%) of three commercial ELISAs for detecting past infection. Nevertheless, all assays had good classification potential but cut-off adaptation is needed. Based on the unequal worldwide distribution of the differently performing tests in studies, Q fever seroprevalence is likely to be underestimated in studies from Europe whereas the data from North America and Australia are likely to be more reliable.
近期，Q热的大规模爆发提高了人们对该疾病作为一个公共卫生问题的认识。对Q热总体影响的了解主要依赖于血清阳性率研究，准确评估血清阳性率至关重要。因此，我们评估了市面上少数几种用于此目的的酶联免疫吸附测定（ELISA）方法。2005年在耶拿（一个拥有10万居民的城市）爆发的疫情为我们提供了评估的机会。然而，我们发现三种用于检测既往感染的商业ELISA方法中的两种（灵敏度分别为42%和51%）令人失望地低。尽管如此，所有检测方法都有良好的分类潜力，但需要调整临界值。基于不同性能检测方法在全球分布不均，欧洲研究中的Q热血清阳性率可能被低估，而北美和澳大利亚的数据可能更可靠。
