Zhou Nan, Zhang Yu, Liu Fang, Cai Lei
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Mycologia. 2016 Jan-Feb;108(1):179-91. doi: 10.3852/15-086. Epub 2015 Oct 21.
This study introduces three new Gymnoascus species (Gymnoascaceae, Onygenales), G. halophilus, G. stercorarius and G. thermotolerans, isolated from sediments in Chaka Salt Lake, compost and cornfield soil, respectively, in China, based on a polyphasic characterization including morphology, physiology and molecular phylogeny. Phylogenetic relationships were assessed based on the nuclear internal transcribed spacer (ITS = ITS1 + 5.8S + ITS2) region and a combined multilocus alignment of the ITS, 18S subunit rRNA gene and 28S subunit rRNA genes. Our study identified phylogenetic and phenotypic characters that differentiated the three new species from known species in the genus. Salinity and temperature tolerance tests revealed that G. halophilus was an obligate halophile while G. stercorarius and G. thermotolerans were halotolerant and thermotolerant. A key to accepted species of Gymnoascus is provided.
本研究基于多相特征描述，包括形态学、生理学和分子系统发育，介绍了从中国茶卡盐湖沉积物、堆肥和玉米田土壤中分别分离出的三个新的裸囊菌属物种（裸囊菌科，爪甲团囊菌目），即嗜盐裸囊菌、粪生裸囊菌和耐热裸囊菌。基于核内转录间隔区（ITS = ITS1 + 5.8S + ITS2）区域以及ITS、18S亚基rRNA基因和28S亚基rRNA基因的联合多位点比对来评估系统发育关系。我们的研究确定了将这三个新物种与该属已知物种区分开来的系统发育和表型特征。盐度和温度耐受性测试表明，嗜盐裸囊菌是专性嗜盐菌，而粪生裸囊菌和耐热裸囊菌具有耐盐性和耐热性。提供了裸囊菌属已接受物种的检索表。
