López-Nevot M A, Esteban F, Ferrón A, Gutiérrez J, Oliva M R, Romero C, Huelin C, Ruiz-Cabello F, Garrido F
Servicio de Análisis Clínicos, Hospital Virgen de las Nieves, Granada, Spain.
Br J Cancer. 1989 Feb;59(2):221-6. doi: 10.1038/bjc.1989.45.
The expression of HLA class I antigens was studied in 99 primary tumour (colorectal, gastric and laryngeal carcinomas) and 57 autologous metastases using immunohistological techniques and monoclonal antibodies against class I monomorphic determinants, HLA-B isotypic determinants and HLA polymorphic determinants. Fourteen per cent of colorectal, 9.6% of gastric and 20% of laryngeal carcinomas completely lacked class I molecules. Selective losses of HLA-B antigens were also detected in 8.8, 3.4 and 5.8% of these tumours respectively. Taking into account complete and selective loss of HLA-B the average alteration in the class I molecules expression totalled 21%. The comparison of class I expression between primary tumours and autologous metastases showed differences in 24% of the patients. These differences consisted mainly in a decrease of class I expression by metastases. Nevertheless, four types of divergence were detected in laryngeal carcinomas, namely: +/-, +/+, -/+, -/-. In addition, a clear correlation between degree of differentiation and class I expression was observed in laryngeal tumours. Finally, when class I gene RFLPs were compared with DNA from 15 tumours and autologous normal mucosa or peripheral lymphocytes, no differences were detected between these samples.
利用免疫组织学技术以及针对I类单态决定簇、HLA - B同种型决定簇和HLA多态决定簇的单克隆抗体，对99例原发性肿瘤（结直肠癌、胃癌和喉癌）和57例自体转移瘤中的HLA I类抗原表达进行了研究。14%的结直肠癌、9.6%的胃癌和20%的喉癌完全缺乏I类分子。在这些肿瘤中，还分别检测到8.8%、3.4%和5.8%的肿瘤存在HLA - B抗原的选择性缺失。将HLA - B的完全缺失和选择性缺失考虑在内，I类分子表达的平均改变总计为21%。原发性肿瘤与自体转移瘤之间I类表达的比较显示，24%的患者存在差异。这些差异主要表现为转移瘤中I类表达的降低。然而，在喉癌中检测到四种差异类型，即：+/-、+/+、-/+、-/-。此外，在喉肿瘤中观察到分化程度与I类表达之间存在明显相关性。最后，当将I类基因RFLP与来自15个肿瘤及自体正常黏膜或外周淋巴细胞的DNA进行比较时，在这些样本之间未检测到差异。
