Ubulom Peace Mayen Edwin, Udobi Chinweizu Ejikeme, Madu Mark Iheukwumere
Pharmaceutical Microbiology and Parasitology Unit, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo, Uyo, Akwa Ibom, Nigeria.
J Trop Med. 2015;2015:947390. doi: 10.1155/2015/947390. Epub 2015 Sep 29.
Objective. The study was designed to determine the efficacy of combined Amodiaquine and Ciprofloxacin in plasmodiasis therapy. Method. The in vivo antiplasmodial effect of different dosage levels of Amodiaquine, Ciprofloxacin, and their combinations against Plasmodium berghei berghei was evaluated using Swiss albino mice. Results. Amodiaquine (a known antiplasmodial agent) had a fairly significant antiplasmodial effect reducing the parasites for every 100 red blood cells (RBC) from 66 to 16 (75.75%) at the tolerable dosage level of 7.5 mg/kg body weight while Ciprofloxacin (an antibiotic known to have antimalarial effect) showed an insignificant antiplasmodial effect reducing the parasites for every 100 RBC from 65 to 64 (1.53%) at the tolerable dosage level of 10.7 mg/kg body weight. Conversely, the combination therapy of Amodiaquine and Ciprofloxacin had a significant antiplasmodial effect at all the doses administered. The combination of 7.5 mg/kg of Amodiaquine and 12.8 mg/kg of Ciprofloxacin, however, showed the most significant antiplasmodial effect of the doses used reducing the number of parasites per 100 RBC from 60 to 10 (83.33%). Conclusions. Appropriate Amodiaquine and Ciprofloxacin combination will be effective for the treatment of malaria and better than either Amodiaquine or Ciprofloxacin singly at their recommended dosage levels.
目的。本研究旨在确定阿莫地喹与环丙沙星联合用药治疗疟原虫病的疗效。方法。使用瑞士白化小鼠评估不同剂量水平的阿莫地喹、环丙沙星及其组合对伯氏疟原虫的体内抗疟原虫作用。结果。阿莫地喹(一种已知的抗疟原虫药物)具有相当显著的抗疟原虫作用,在7.5毫克/千克体重的耐受剂量水平下,每100个红细胞(RBC)中的疟原虫数量从66降至16(75.75%);而环丙沙星(一种已知具有抗疟作用的抗生素)在10.7毫克/千克体重的耐受剂量水平下,抗疟原虫作用不显著,每100个RBC中的疟原虫数量仅从65降至64(1.53%)。相反,阿莫地喹与环丙沙星的联合疗法在所有给药剂量下均具有显著的抗疟原虫作用。然而,7.5毫克/千克的阿莫地喹与12.8毫克/千克的环丙沙星组合在所用剂量中显示出最显著的抗疟原虫作用,每100个RBC中的疟原虫数量从60降至10(83.33%)。结论。适当的阿莫地喹与环丙沙星组合对疟疾治疗有效,且在推荐剂量水平下比单独使用阿莫地喹或环丙沙星效果更好。