Sangha Jatinder Singh, Wally Owen, Banskota Arjun H, Stefanova Roumiana, Hafting Jeff T, Critchley Alan T, Prithiviraj Balakrishnan
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada.
Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada.
Mar Drugs. 2015 Oct 20;13(10):6407-24. doi: 10.3390/md13106407.
We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against β-amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human Aβ1-42 gene. The methanol extract of C. crispus (CCE), delayed β-amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of Aβ species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of β-amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against β-amyloid-induced toxicity in C. elegans, partly through reduced β-amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS).
我们在此报告，在表达人Aβ1-42基因的转基因秀丽隐杆线虫中，养殖的红海藻皱波角叉菜（Chondrus crispus）的甲醇提取物对β-淀粉样蛋白诱导的毒性具有保护作用。皱波角叉菜的甲醇提取物（CCE）可延缓β-淀粉样蛋白诱导的麻痹，而水提取物（CCW）则无效。CCE处理不影响amy1的转录丰度；然而，蛋白质免疫印迹分析显示，与未处理的线虫相比，Aβ种类显著减少。在经CCE处理的线虫中，应激反应基因sod3、hsp16.2和skn1的转录丰度增加。对CCE进行生物测定指导的分级分离，得到了富含单半乳糖基二酰基甘油（MGDG）的级分，该级分显著延缓了β-淀粉样蛋白诱导的麻痹的发作。综上所述，这些结果表明，养殖的皱波角叉菜菌株在提供膳食营养价值的同时，可能还对秀丽隐杆线虫中β-淀粉样蛋白诱导的毒性具有显著的保护作用，部分原因是通过减少β-淀粉样蛋白种类、上调应激诱导基因以及减少活性氧（ROS）的积累。
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