Chen Tian-ling, Bi Chang-qiong, Xiao Xue, Ma Xian-peng, Ge Fa-huan
Zhong Yao Cai. 2015 Mar;38(3):536-9.
To establish the HPLC fingerprint of water-soluble components of Salvia miltiorrhiza in Songtao, Guizhou, and to perform simultaneous determination of six components in it, so as to provide analytical method for its quality control.
The analyses were performed on a Phenomenex Luna C18 (2) (250 mm x 4. 6 mm, 5µm) column eluted with 0. 4% formic acid(A) - acetonitrile(B) in a gradient mode. The flow rate was 1. 0 mL/min, column temperature was set at 30 °C.
Eleven common peaks were identified form the HPLC fingerprint of Salvia miltiorrhiza from 10 batches, the HPLC fingerprint similarities of 10 batches were not less than 0. 999. The linear ranges of danshensu, protocatechuic aldehyde, caffeic acid, rosmarinic acid, lithospermic acid and salvianolic acid B were 0. 0680 ~ 1. 3583 mg/mL, 0. 0008 ~ 0. 3967 mg/mL, 0. 0005 ~ 0. 2660 mg/mL, 0. 0020 ~ 0. 3992 mg/mL, 0. 0063 ~ 0. 6311 mg/mL and 0. 0097 ~ 1. 9306 mg/mL with r ≥ 0. 9999, respectively. The recovery rates were 100. 84%,102. 44%, 100. 53% ,100. 63%, 100. 83% and 100. 35% with RSD <2. 3%, respectively.
The established method is simple, accurate and can provide reference for quality control of Salvia miltiorrhiza.
建立贵州松桃丹参水溶性成分的高效液相色谱指纹图谱，并同时测定其中6种成分，为其质量控制提供分析方法。
采用Phenomenex Luna C18(2)(250 mm×4.6 mm，5μm)色谱柱，以0.4%甲酸(A)-乙腈(B)为流动相进行梯度洗脱。流速为1.0 mL/min，柱温设定为30℃。
从10批丹参的高效液相色谱指纹图谱中鉴定出11个共有峰，10批样品的高效液相色谱指纹图谱相似度均不低于0.999。丹参素、原儿茶醛、咖啡酸、迷迭香酸、紫草酸、丹酚酸B的线性范围分别为0.0680～1.3583 mg/mL、0.0008～0.3967 mg/mL、0.0005～0.2660 mg/mL、0.0020～0.3992 mg/mL、0.0063～0.6311 mg/mL、0.0097～1.9306 mg/mL，r≥0.9999。加样回收率分别为100.84%、102.44%、100.53%、100.63%、100.83%、100.35%，RSD<2.3%。
所建立的方法简便、准确，可为丹参的质量控制提供参考。
