Li J, He L Y, Song W Z
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1993;28(7):543-7.
A new analytical method for the separation and determination of seven aqueous depsides in salvia miltiorrhiza by HPTLC has been developed. The seven depsides are protocatechualdehyde, caffeic acid, methyl rosmarinate, rosmarinic acid, salvianolic acid A, B and C. Using chloroform-ethyl acetate-benzene-formic acid(2.4:2:1:0.6) as developing solvent A, protocatechualdehyde was separated; using chloroform-ethyl acetate-benzene-formic acid-methanol (1.5:2:1:1:0.1) as developing solvent B, the other six constituents were well separated. The aqueous depsides were detected at the wave lengths of lambda S = 300 nm and lambda R = 240. This method is simple, rapid, sensitive and accurate. The contents of seven depsides in several Salvia species were determined.
已开发出一种采用高效薄层色谱法分离和测定丹参中七种水溶性缩酚酸的新分析方法。这七种缩酚酸分别是原儿茶醛、咖啡酸、迷迭香酸甲酯、迷迭香酸、丹酚酸A、B和C。以氯仿 - 乙酸乙酯 - 苯 - 甲酸（2.4:2:1:0.6）作为展开剂A，可分离出原儿茶醛；以氯仿 - 乙酸乙酯 - 苯 - 甲酸 - 甲醇（1.5:2:1:1:0.1）作为展开剂B，可将其他六种成分良好分离。水溶性缩酚酸在λS = 300 nm和λR = 240波长处进行检测。该方法简便、快速、灵敏且准确。已测定了几种丹参属植物中七种缩酚酸的含量。
