Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Biosens Bioelectron. 2016 Mar 15;77:702-8. doi: 10.1016/j.bios.2015.10.043. Epub 2015 Oct 23.
The number of species and planting areas of genetically modified organisms (GMOs) has been rapidly developed during the past ten years. For the purpose of GMO inspection, quarantine and manipulation, we have now devised a high-throughput Bt-based GMOs screening method based on the liquid bead array. This novel method is based on the direct competitive recognition between biotinylated antibodies and beads-coupled antigens, searching for Bt presence in samples if it contains Bt Cry1 Aa, Bt Cry1 Ab, Bt Cry1 Ac, Bt Cry1 Ah, Bt Cry1 B, Bt Cry1 C, Bt Cry1 F, Bt Cry2 A, Bt Cry3 or Bt Cry9 C. Our method has a wide GMO species coverage so that more than 90% of the whole commercialized GMO species can be identified throughout the world. Under our optimization, specificity, sensitivity, repeatability and availability validation, the method shows a high specificity and 10-50 ng/mL sensitivity of quantification. We then assessed more than 1800 samples in the field and food market to prove capacity of our method in performing a high throughput screening work for GMO manipulation. Our method offers an applicant platform for further inspection and research on GMO plants.
在过去的十年中，转基因生物（GMO）的种类和种植面积迅速发展。为了进行 GMO 的检测、检疫和操作，我们现在设计了一种基于液珠阵列的高通量 Bt 基 GMO 筛选方法。这种新方法基于生物素化抗体与珠偶联抗原之间的直接竞争性识别，如果样品中含有 Bt Cry1Aa、Bt Cry1Ab、Bt Cry1Ac、Bt Cry1Ah、Bt Cry1B、Bt Cry1C、Bt Cry1F、Bt Cry2A、Bt Cry3 或 Bt Cry9C，则可以检测到 Bt 的存在。我们的方法具有广泛的 GMO 物种覆盖范围，因此可以识别全世界 90%以上的商业化 GMO 物种。在我们的优化下，该方法具有很高的特异性和 10-50ng/mL 的定量灵敏度，通过特异性、灵敏度、重复性和可用性验证。然后，我们评估了 1800 多个田间和食品市场的样本，以证明我们的方法在进行 GMO 操作的高通量筛选方面的能力。我们的方法为进一步检查和研究 GMO 植物提供了一个应用平台。
