Sunar Serap, Yildirim Nalan, Aksakal Ozkan, Agar Guleray
Department of Biology, Atatürk University, Erzurum, Turkey.
Toxicol Ind Health. 2013 Jun;29(5):449-59. doi: 10.1177/0748233712436644. Epub 2012 Feb 23.
In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75 and 100 μl concentrations and applied to corn seed. The total soluble protein and genomic template stability results were compared with the control. The results showed that especially 100 μl extracts of diluted leaf, stem and root had a strong inhibitory activity on the genomic template stability. The changes occurred in random amplification of polymorphic DNA (RAPD) profiles of C. arvensis extract treatment included variation in band intensity, loss of bands and appearance of new bands compared with control. Also, the results obtained from this study revealed that the increase in the concentrations of C. arvensis extract increased the total soluble protein content in maize. The results suggested that RAPD analysis and total protein analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals.
在本研究中，对田旋花的甲醇提取物进行了遗传毒性测试，并检测了其对玉米种子总可溶性蛋白含量和基因组模板稳定性的抑制活性。将田旋花的叶、茎和根的甲醇提取物稀释至50、75和100微升浓度，并应用于玉米种子。将总可溶性蛋白和基因组模板稳定性结果与对照进行比较。结果表明，特别是稀释后的叶、茎和根的100微升提取物对基因组模板稳定性具有很强的抑制活性。与对照相比，田旋花提取物处理的多态性DNA（RAPD）图谱随机扩增中出现的变化包括条带强度变化、条带缺失和新条带出现。此外，本研究获得的结果表明，田旋花提取物浓度的增加会使玉米中的总可溶性蛋白含量增加。结果表明，RAPD分析和总蛋白分析可作为检测植物化感物质遗传毒性效应的合适生物标志物检测方法。
