二次谐波产生显微镜对受水合作用影响的三种大麦淀粉系晶体超微结构的研究。
Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration.
作者信息
Cisek Richard, Tokarz Danielle, Steup Martin, Tetlow Ian J, Emes Michael J, Hebelstrup Kim H, Blennow Andreas, Barzda Virginijus
机构信息
Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Department of Plant Physiology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25 Building 20, 14476 Potsdam, Germany ; Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
出版信息
Biomed Opt Express. 2015 Sep 1;6(10):3694-700. doi: 10.1364/BOE.6.003694. eCollection 2015 Oct 1.
Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'SHG ) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.
二次谐波产生（SHG）显微镜用于研究由于大麦淀粉颗粒中基因表达和水合作用改变而导致的晶体结构变化。使用简化的斯托克斯-穆勒偏振显微镜获得SHG强度和磁化率比值（R'SHG）。最大R'SHG值出现在中等湿度下，表明来自葡聚糖螺旋圆柱轴的非线性偶极子的取向分布最窄。最大SHG强度出现在最高湿度和支链淀粉含量时。这些结果支持了以下假设：SHG是由有序的氢键和羟基键网络引起的，这些网络随着淀粉颗粒的水合作用而增加。
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