• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

二次谐波产生显微镜：一种用于生物成像的强大工具。

Second harmonic generation microscopy: a powerful tool for bio-imaging.

作者信息

Aghigh Arash, Bancelin Stéphane, Rivard Maxime, Pinsard Maxime, Ibrahim Heide, Légaré François

机构信息

Centre Énergie Matériaux Télécommunications, Institut National de La Recherche Scientifique, Varennes, QC Canada.

Univ. Bordeaux, CNRS, IINS, UMR 5297, 33000 Bordeaux, France.

出版信息

Biophys Rev. 2023 Jan 19;15(1):43-70. doi: 10.1007/s12551-022-01041-6. eCollection 2023 Feb.

DOI:10.1007/s12551-022-01041-6
PMID:36909955
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9995455/
Abstract

Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique.

摘要

二次谐波产生（SHG）显微镜术是一种在多种应用中都很重要的光学成像技术。本文描述了SHG显微镜术及其更先进变体的历史和物理原理，以及它们在生物医学应用中的优缺点。它还概述了神经科学和微管成像中的SHG及先进SHG成像，以及这些方法如何有助于理解微管的形成、结构以及在神经元功能中的作用。最后，我们对这些方法的未来以及技术进步如何有助于使SHG显微镜术成为一种更广泛应用的成像技术提出了展望。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/55efea39f238/12551_2022_1041_Fig21_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/d21c52acce3a/12551_2022_1041_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4ffc229c9dcc/12551_2022_1041_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/395b3fc694c5/12551_2022_1041_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/872fe79cb878/12551_2022_1041_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/6e785db5e9a2/12551_2022_1041_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/789d6daea5ed/12551_2022_1041_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/36cbd8696494/12551_2022_1041_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/20b852c268bd/12551_2022_1041_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4eb15c018d1f/12551_2022_1041_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/40df64475228/12551_2022_1041_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/bf65bb070199/12551_2022_1041_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/e4522ec5813c/12551_2022_1041_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4682cfe941d1/12551_2022_1041_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/8204bf65511a/12551_2022_1041_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/b566b2d4dc02/12551_2022_1041_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/58a7d2c148e8/12551_2022_1041_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/1cf46a4bb59e/12551_2022_1041_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/940d006b5a13/12551_2022_1041_Fig18_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/337afdac2842/12551_2022_1041_Fig19_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/5b5403bee9c6/12551_2022_1041_Fig20_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/55efea39f238/12551_2022_1041_Fig21_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/d21c52acce3a/12551_2022_1041_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4ffc229c9dcc/12551_2022_1041_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/395b3fc694c5/12551_2022_1041_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/872fe79cb878/12551_2022_1041_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/6e785db5e9a2/12551_2022_1041_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/789d6daea5ed/12551_2022_1041_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/36cbd8696494/12551_2022_1041_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/20b852c268bd/12551_2022_1041_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4eb15c018d1f/12551_2022_1041_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/40df64475228/12551_2022_1041_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/bf65bb070199/12551_2022_1041_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/e4522ec5813c/12551_2022_1041_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/4682cfe941d1/12551_2022_1041_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/8204bf65511a/12551_2022_1041_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/b566b2d4dc02/12551_2022_1041_Fig15_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/58a7d2c148e8/12551_2022_1041_Fig16_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/1cf46a4bb59e/12551_2022_1041_Fig17_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/940d006b5a13/12551_2022_1041_Fig18_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/337afdac2842/12551_2022_1041_Fig19_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/5b5403bee9c6/12551_2022_1041_Fig20_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca3b/9995648/55efea39f238/12551_2022_1041_Fig21_HTML.jpg

相似文献

1
Second harmonic generation microscopy: a powerful tool for bio-imaging.二次谐波产生显微镜：一种用于生物成像的强大工具。
Biophys Rev. 2023 Jan 19;15(1):43-70. doi: 10.1007/s12551-022-01041-6. eCollection 2023 Feb.
2
Super-Resolution Second-Harmonic Generation Imaging with Multifocal Structured Illumination Microscopy.基于多点结构光照明显微镜的超分辨率二次谐波成像
Nano Lett. 2023 Sep 13;23(17):7975-7982. doi: 10.1021/acs.nanolett.3c01903. Epub 2023 Aug 29.
3
Second harmonic generation microscopy using pixel reassignment.基于像素重分配的二次谐波产生显微镜技术
J Microsc. 2021 Jan;281(1):97-105. doi: 10.1111/jmi.12956. Epub 2020 Sep 2.
4
Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.光学谐波产生显微镜的最新进展：应用与展望
BME Front. 2021 Jan 25;2021:3973857. doi: 10.34133/2021/3973857. eCollection 2021.
5
Probing Collagen Organization: Practical Guide for Second-Harmonic Generation (SHG) Imaging.探究胶原蛋白组织：二次谐波产生（SHG）成像实用指南
Methods Mol Biol. 2017;1627:409-425. doi: 10.1007/978-1-4939-7113-8_27.
6
Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy.利用多光子激发荧光和偏振敏感谐波产生显微镜对胰腺癌组织进行表征
Front Oncol. 2019 Apr 17;9:272. doi: 10.3389/fonc.2019.00272. eCollection 2019.
7
Elimination of imaging artifacts in second harmonic generation microscopy using interferometry.利用干涉测量法消除二次谐波产生显微镜中的成像伪影。
Biomed Opt Express. 2019 Jul 12;10(8):3938-3952. doi: 10.1364/BOE.10.003938. eCollection 2019 Aug 1.
8
Ultrasensitive Three-Dimensional Orientation Imaging of Single Molecules on Plasmonic Nanohole Arrays Using Second Harmonic Generation.利用二次谐波产生技术在等离子体纳米孔阵列上对单个分子进行超灵敏三维取向成像。
Nano Lett. 2019 Sep 11;19(9):6192-6202. doi: 10.1021/acs.nanolett.9b02239. Epub 2019 Aug 12.
9
Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.二次谐波产生成像显微镜在卵巢癌和乳腺癌中的应用。
Perspect Medicin Chem. 2015 Apr 16;7:21-32. doi: 10.4137/PMC.S13214. eCollection 2015.
10
Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.谐波产生显微镜2.0：助力神经科学活体成像的新方法
Front Mol Biosci. 2019 Oct 9;6:99. doi: 10.3389/fmolb.2019.00099. eCollection 2019.

引用本文的文献

1
Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation.利用双通道双光子和二次谐波激发对微小样本中的器官纤维化进行早期非线性成像检测
Biosensors (Basel). 2025 Jun 4;15(6):357. doi: 10.3390/bios15060357.
2
Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis.体外模拟肿瘤微环境复杂性：球体作为生理相关肿瘤模型及其分析策略
Cells. 2025 May 17;14(10):732. doi: 10.3390/cells14100732.
3
Photon-photon chemical thermodynamics of frequency conversion processes in highly multimode systems.

本文引用的文献

1
Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.光学谐波产生显微镜的最新进展：应用与展望
BME Front. 2021 Jan 25;2021:3973857. doi: 10.34133/2021/3973857. eCollection 2021.
2
H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy.无标记二次谐波产生显微镜揭示 H-ABC 微管蛋白病。
Sci Rep. 2022 Aug 24;12(1):14417. doi: 10.1038/s41598-022-18370-x.
3
Phosphorylation of eIF4E in the stroma drives the production and spatial organisation of collagen type I in the mammary gland.
高多模系统中频率转换过程的光子 - 光子化学热力学
Light Sci Appl. 2025 May 12;14(1):188. doi: 10.1038/s41377-025-01856-4.
4
Intravital Imaging of Immune Responses in the Cancer Microenvironment.癌症微环境中免疫反应的活体成像
Cancer Med. 2025 Apr;14(8):e70899. doi: 10.1002/cam4.70899.
5
How to Study the Mechanobiology of Intestinal Epithelial Organoids? A Review of Culture Supports, Imaging Techniques, and Analysis Methods.如何研究肠道上皮类器官的力学生物学？培养支架、成像技术及分析方法综述
Biol Cell. 2025 Apr;117(4):e70003. doi: 10.1111/boc.70003.
6
The Micro-Structure of the Celiac Ganglia-A Two-Photon Microscopy Study on Parkinson's Disease.腹腔神经节的微观结构——一项关于帕金森病的双光子显微镜研究
Diagnostics (Basel). 2025 Mar 8;15(6):659. doi: 10.3390/diagnostics15060659.
7
Advances in Extracellular Matrix-Associated Diagnostics and Therapeutics.细胞外基质相关诊断与治疗的进展
J Clin Med. 2025 Mar 10;14(6):1856. doi: 10.3390/jcm14061856.
8
Impact of paraben on uterine collagen: An integrated and targeted Correlative approach using second harmonic generation microscopy, nanoindentation, and atomic force microscopy.对羟基苯甲酸酯对子宫胶原蛋白的影响：使用二次谐波产生显微镜、纳米压痕和原子力显微镜的综合靶向相关方法。
J Mech Behav Biomed Mater. 2025 May;165:106926. doi: 10.1016/j.jmbbm.2025.106926. Epub 2025 Feb 1.
9
A gene-encoded bioprotein second harmonic generation (SHG) probe from Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin for live cell imaging.一种来自苜蓿银纹夜蛾核型多角体病毒（AcMNPV）多角体蛋白的基因编码生物蛋白二次谐波产生（SHG）探针，用于活细胞成像。
Eur Biophys J. 2025 Feb;54(1-2):21-32. doi: 10.1007/s00249-024-01728-6. Epub 2025 Jan 15.
10
Open quantum systems theory of ultraweak ultraviolet photon emissions: Revisiting Gurwitsch's onion experiment as a prototype for quantum biology.超微弱紫外线光子发射的开放量子系统理论：重新审视古尔维奇的洋葱实验作为量子生物学的一个原型
Comput Struct Biotechnol J. 2024 Nov 29;26:78-91. doi: 10.1016/j.csbj.2024.11.030. eCollection 2024 Dec.
基质中真核翻译起始因子4E（eIF4E）的磷酸化驱动乳腺中I型胶原蛋白的产生和空间组织。
Matrix Biol. 2022 Aug;111:264-288. doi: 10.1016/j.matbio.2022.07.003. Epub 2022 Jul 14.
4
Second harmonic generation microscopy of otoconia.耳石的二次谐波产生显微镜检查
Biomed Opt Express. 2022 May 26;13(6):3593-3600. doi: 10.1364/BOE.457967. eCollection 2022 Jun 1.
5
Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy.基于宽场偏振二次谐波显微镜的机器学习癌症诊断
Sci Rep. 2022 Jun 18;12(1):10290. doi: 10.1038/s41598-022-13623-1.
6
Two-Photon Endoscopy: State of the Art and Perspectives.双光子内窥镜：现状与展望。
Mol Imaging Biol. 2023 Feb;25(1):3-17. doi: 10.1007/s11307-021-01665-2. Epub 2021 Nov 15.
7
Quantitative structural imaging of keratoconic corneas using polarization-resolved SHG microscopy.使用偏振分辨二次谐波显微镜对圆锥角膜进行定量结构成像。
Biomed Opt Express. 2021 Jun 16;12(7):4163-4178. doi: 10.1364/BOE.426145. eCollection 2021 Jul 1.
8
Label-Free Multiphoton Microscopy: Much More Than Fancy Images.无标记多光子显微镜：不仅仅是花哨的图像。
Int J Mol Sci. 2021 Mar 6;22(5):2657. doi: 10.3390/ijms22052657.
9
Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy.偏振分辨二次谐波成像作为一种快速筛选衰老过程中胶原蛋白变化的方法：与光镜和电镜的比较。
J Biophotonics. 2021 Mar;14(3):e202000362. doi: 10.1002/jbio.202000362. Epub 2020 Dec 1.
10
Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization.基于斯托克斯偏振的二次谐波产生显微镜用于胶原和骨骼肌纤维特征分析。
Lasers Med Sci. 2021 Aug;36(6):1161-1167. doi: 10.1007/s10103-020-03144-6. Epub 2020 Sep 18.