Aghigh Arash, Bancelin Stéphane, Rivard Maxime, Pinsard Maxime, Ibrahim Heide, Légaré François
Centre Énergie Matériaux Télécommunications, Institut National de La Recherche Scientifique, Varennes, QC Canada.
Univ. Bordeaux, CNRS, IINS, UMR 5297, 33000 Bordeaux, France.
Biophys Rev. 2023 Jan 19;15(1):43-70. doi: 10.1007/s12551-022-01041-6. eCollection 2023 Feb.
Second harmonic generation (SHG) microscopy is an important optical imaging technique in a variety of applications. This article describes the history and physical principles of SHG microscopy and its more advanced variants, as well as their strengths and weaknesses in biomedical applications. It also provides an overview of SHG and advanced SHG imaging in neuroscience and microtubule imaging and how these methods can aid in understanding microtubule formation, structuration, and involvement in neuronal function. Finally, we offer a perspective on the future of these methods and how technological advancements can help make SHG microscopy a more widely adopted imaging technique.
二次谐波产生（SHG）显微镜术是一种在多种应用中都很重要的光学成像技术。本文描述了SHG显微镜术及其更先进变体的历史和物理原理，以及它们在生物医学应用中的优缺点。它还概述了神经科学和微管成像中的SHG及先进SHG成像，以及这些方法如何有助于理解微管的形成、结构以及在神经元功能中的作用。最后，我们对这些方法的未来以及技术进步如何有助于使SHG显微镜术成为一种更广泛应用的成像技术提出了展望。
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