Benedict John C
Department of Earth and Environmental Sciences, University of Michigan, 2534 CC Little Building, 1100 North University Avenue, Ann Arbor, Michigan 48109-1005 USA.
Appl Plant Sci. 2015 Oct 12;3(10). doi: 10.3732/apps.1500075. eCollection 2015 Oct.
A novel preparation technique was developed to examine fruits and seeds of plants with exceptionally hard or brittle tissues that are very difficult to prepare using standard histological techniques.
The method introduced here was modified from a technique employed on fossil material and has been adapted for use on fruits and seeds of extant plants. A variety of fruits and seeds have been prepared with great success, and the technique will be useful for any excessively hard fruits or seeds that are not able to be prepared using traditional embedding or sectioning methods.
When compared to existing techniques for obtaining anatomical features of fruits and seeds, the protocol described here has the potential to create high-quality thin sections of materials that are not able to be sectioned using traditional histological techniques, which can be produced quickly and without the need for harmful chemicals.
开发了一种新的制备技术，用于检查具有异常坚硬或脆硬组织的植物果实和种子，这些组织使用标准组织学技术很难制备。
此处介绍的方法是对用于化石材料的技术进行修改后得到的，并已适用于现存植物的果实和种子。已经成功制备了多种果实和种子，该技术将适用于任何无法使用传统包埋或切片方法制备的过硬果实或种子。
与现有的获取果实和种子解剖特征的技术相比，本文所述方案有可能创建高质量的薄切片，这些材料无法使用传统组织学技术切片，而且可以快速制备，无需使用有害化学物质。
