一种用于子宫内膜样本中GALR1定量甲基化分析的焦磷酸测序检测法：初步结果

A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results.

作者信息

Kottaridi Christine, Koureas Nikolaos, Margari Niki, Terzakis Emmanouil, Bilirakis Evripidis, Pappas Asimakis, Chrelias Charalampos, Spathis Aris, Aga Evangelia, Pouliakis Abraham, Panayiotides Ioannis, Karakitsos Petros

机构信息

Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.

2nd Department of Gynecology, St. Savas Cancer Hospital, 171 Alexandras Avenue, 11522 Athens, Greece.

出版信息

Biomed Res Int. 2015;2015:756359. doi: 10.1155/2015/756359. Epub 2015 Oct 4.

DOI:10.1155/2015/756359

PMID:26504828

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4609388/

Abstract

Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin's expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin's receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis of GALR1 promoter can be a useful ancillary marker to cytology as the histological status can successfully predict. This marker has the potential to lead towards better management of women with endometrial lesions and eventually reduce unnecessary interventions. In addition it can provide early warning for women with negative cytological result.

摘要

子宫内膜癌是女性生殖道最常见的恶性肿瘤，而异常的DNA甲基化似乎在子宫内膜癌发生过程中起关键作用。甘丙肽的表达与多种癌症有关。我们开发了一种新的焦磷酸测序检测方法，用于定量甘丙肽受体-1（GALR1）的DNA甲基化。在本研究中，初步结果表明，GALR1启动子的焦磷酸测序甲基化分析可作为细胞学的有用辅助标志物，因为组织学状态能够成功预测。该标志物有可能使子宫内膜病变女性得到更好的管理，并最终减少不必要的干预。此外，它还可为细胞学结果阴性的女性提供早期预警。