倍他米松用于预期早产时的类固醇途径基因与新生儿呼吸窘迫
Steroid Pathway Genes and Neonatal Respiratory Distress After Betamethasone Use in Anticipated Preterm Birth.
作者信息
Haas David M, Lai Dongbing, Sharma Sunita, Then Jenny, Kho Alvin, Flockhart David A, Tantisira Kelan, Foroud Tatiana
机构信息
Indiana University School of Medicine, Indianapolis, IN, USA
Indiana University School of Medicine, Indianapolis, IN, USA.
出版信息
Reprod Sci. 2016 May;23(5):680-6. doi: 10.1177/1933719115612129. Epub 2015 Oct 27.
OBJECTIVE
To test several key glucocorticoid genes that are enhanced in lung development for associations with respiratory distress syndrome (RDS) after antenatal corticosteroid use.
METHODS
A prospective cohort of women received betamethasone to accelerate fetal lung maturity for threatened preterm delivery. DNA was obtained from mothers and newborns. Neonatal RDS was the primary outcome. Genotyping for single-nucleotide polymorphisms (SNPs) in 68 glucocorticoid genes found to be differentially expressed during lung development was performed. Multivariable analysis tested for associations of SNPs in the candidate genes with RDS.
RESULTS
Genotypic results for 867 SNPs in 96 mothers and 73 babies were included. Thirty-nine (53.4%) babies developed RDS. Maternal SNPs in the centromeric protein E (CENPE), GLRX, CD9, and AURKA genes provided evidence of association with RDS (P < .01). In newborns, SNPs in COL4A3, BHLHE40, and SRGN provided evidence of association with RDS (P < .01).
CONCLUSION
Single-nucleotide polymorphisms in several glucocorticoid responsive genes suggest association with neonatal RDS after antenatal corticosteroid use.
目的
检测在肺发育过程中表达增强的几个关键糖皮质激素基因，以探究其与产前使用糖皮质激素后发生呼吸窘迫综合征（RDS）之间的关联。
方法
一项前瞻性队列研究中，对因有早产风险而接受倍他米松以促进胎儿肺成熟的女性进行研究。从母亲和新生儿处获取DNA。新生儿RDS为主要观察指标。对68个在肺发育过程中差异表达的糖皮质激素基因进行单核苷酸多态性（SNP）基因分型。多变量分析检测候选基因中的SNP与RDS的关联。
结果
纳入了96名母亲和73名婴儿的867个SNP的基因分型结果。39名（53.4%）婴儿发生了RDS。着丝粒蛋白E（CENPE）、谷氧还蛋白（GLRX）、CD9和极光激酶A（AURKA）基因中的母亲SNP提供了与RDS相关的证据（P < 0.01）。在新生儿中，Ⅳ型胶原α3链（COL4A3）、基本螺旋-环-螺旋家族成员e40（BHLHE40）和分泌型调节蛋白（SRGN）中的SNP提供了与RDS相关的证据（P < 0.01）。
结论
几个糖皮质激素反应基因中的单核苷酸多态性提示与产前使用糖皮质激素后新生儿RDS有关。
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