Armstrong P B
Department of Zoology, University of California, Davis.
Crit Rev Biochem Mol Biol. 1989;24(2):119-49. doi: 10.3109/10409238909086396.
The question posed by the science of analytical histology is how the properties and interactions of the components of the tissues determine their organization in the organs. The relevant components of the tissues are the cells and the extracellular matrix. The ability of cohering populations of cells to self-assemble structured tissues by cell sorting out offers an important opportunity for the experimental study of the mechanisms by which the cells and extracellular matrix interact to determine structure. The investigator can manipulate the initial organization and the cellular composition of the system and, in favorable situations, the composition of the extracellular matrix and the activities of candidate adhesive molecules. It can reasonably be expected that the recent progress in the characterization of the molecular species involved in cell-cell and cell-extracellular matrix interaction will allow the analysis of the molecular basis of tissue organization, with study of the self-assembly of tissue structure during sorting out playing an important role in this analysis. The importance of the differential adhesion hypothesis is its success in describing the rules by which macroscopic tissue structure is governed by the adhesive interactions of cell with cell and cell with extracellular matrix. The DAH describes how the physical forces of cell-cell and cell-matrix adhesion determine structure. Elucidation of the particular adhesive molecules involved in these interactions (e.g., the CAMs, junctional proteins, and matrix adhesion molecules) will yield an explanation at the biochemical level. A complete understanding of structure requires both levels of explanation.
分析组织学这门科学提出的问题是，组织成分的特性和相互作用如何决定它们在器官中的组织方式。组织的相关成分是细胞和细胞外基质。细胞群体通过细胞分选自组装成结构化组织的能力，为研究细胞与细胞外基质相互作用以确定结构的机制提供了重要的实验机会。研究者可以操纵系统的初始组织和细胞组成，在有利的情况下，还可以操纵细胞外基质的组成和候选黏附分子的活性。可以合理预期，近期在表征参与细胞 - 细胞和细胞 - 细胞外基质相互作用的分子种类方面取得的进展，将有助于分析组织组织的分子基础，其中研究分选过程中组织结构的自组装在这一分析中起着重要作用。差异黏附假说的重要性在于它成功地描述了宏观组织结构由细胞与细胞以及细胞与细胞外基质的黏附相互作用所支配的规则。差异黏附假说描述了细胞 - 细胞和细胞 - 基质黏附的物理力如何决定结构。阐明参与这些相互作用的特定黏附分子（例如，细胞黏附分子、连接蛋白和基质黏附分子）将在生化水平上给出解释。对结构的完整理解需要这两个层面的解释。
