Varlamova E G, Novoselov S V, Novoselov V I
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, 142290, Russia.
Mol Biol (Mosk). 2015 Sep-Oct;49(5):785-9. doi: 10.7868/S0026898415050183.
To date various bioinformatics tools allowed to identify 25 selenocysteine-containing mammalian proteins. The name of these proteins assumes that they contain the amino acid selenocysteine (Sec). Functionally characterized selenocysteine-containing proteins are oxidoreductases with various functions, including glutathione peroxidases, thioredoxin reductases, deiodinases etc. However, the functions of more than half of identified proteins are still unclear, and mammalian selenoprotein SeIV is among them. We studied the selV in all stages of postnatal development with the maximum level of mRNA expression during puberty, whereas in adult mice (8-18 months) we observed a gradual decrease of expression. In order to get closer to the functional role of Selenoprotein V, we have carried out experiments on the substrate specificity and enzymatic activity measurement of this selenocysteine-containing protein. It was shown that SelV posseses glutathionperoxidase and thioredoxinreductase activities.
迄今为止，各种生物信息学工具已能识别出25种含硒代半胱氨酸的哺乳动物蛋白质。这些蛋白质的名称表明它们含有氨基酸硒代半胱氨酸（Sec）。已进行功能表征的含硒代半胱氨酸蛋白质是具有多种功能的氧化还原酶，包括谷胱甘肽过氧化物酶、硫氧还蛋白还原酶、脱碘酶等。然而，超过半数已识别蛋白质的功能仍不清楚，哺乳动物硒蛋白SeIV便在其中。我们研究了硒蛋白V在出生后发育各阶段的情况，其mRNA表达在青春期达到最高水平，而在成年小鼠（8 - 18个月）中，我们观察到其表达逐渐下降。为了更深入了解硒蛋白V的功能作用，我们对这种含硒代半胱氨酸蛋白质的底物特异性和酶活性测定进行了实验。结果表明，硒蛋白V具有谷胱甘肽过氧化物酶和硫氧还蛋白还原酶活性。
