硒代半胱氨酸对硒蛋白 S 过氧化物酶活性的贡献。
Contribution of selenocysteine to the peroxidase activity of selenoprotein S.
机构信息
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
出版信息
Biochemistry. 2013 Aug 20;52(33):5514-6. doi: 10.1021/bi400741c. Epub 2013 Aug 12.
Abstract
Selenoprotein S (SelS, VIMP) is an intrinsically disordered enzyme that utilizes selenocysteine to catalyze the reduction of disulfide bonds and peroxides. Here it is demonstrated that selenocysteine is the residue oxidized by the peroxide substrate. It is possible to trap the reaction intermediate selenenic acid when the resolving cysteine is mutated. The selenocysteine allows SelS to rapidly re-form its selenenylsulfide bond following its reduction, and to resist inactivation by H2O2. We propose that SelS's peroxidase mechanism is similar to that of atypical 2-Cys peroxiredoxin and that selenocysteine allows SelS to sustain activity under oxidative stress.
摘要
硒蛋白 S（SelS，VIMP）是一种固有无序的酶，利用硒代半胱氨酸催化二硫键和过氧化物的还原。本文证明了过氧化物底物氧化的残基是硒代半胱氨酸。当解析半胱氨酸发生突变时，可以捕获反应中间体硒代磺酸。硒代半胱氨酸允许 SelS 在还原后快速重新形成其硒代亚磺酰硫键，并抵抗 H2O2 的失活。我们提出 SelS 的过氧化物酶机制类似于非典型 2-Cys 过氧化物酶，并且硒代半胱氨酸允许 SelS 在氧化应激下维持活性。
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Alternative transcripts and 3'UTR elements govern the incorporation of selenocysteine into selenoprotein S.替代转录本和 3'UTR 元件调控硒代半胱氨酸掺入硒蛋白 S。
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The intrinsically disordered membrane protein selenoprotein S is a reductase in vitro.固有无序的膜蛋白硒蛋白 S 是体外的一种还原酶。
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The human selenoprotein VCP-interacting membrane protein (VIMP) is non-globular and harbors a reductase function in an intrinsically disordered region.人源 VCP 相互作用的膜蛋白（VIMP）是非球形的，并在一个无规则卷曲区域中具有还原酶功能。
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