Kim Bong Gyun, Park Hong Woo
Department of Chemical Engineering, Hanyang University, Seoul, 133-791, South Korea.
Appl Microbiol Biotechnol. 2016 Mar;100(5):2163-70. doi: 10.1007/s00253-015-7096-x. Epub 2015 Oct 29.
Effects of high ZnSO4·7H2O supplementation on cell growth and monoclonal antibody (mAb) production in chemically defined suspension cultures of recombinant Chinese hamster ovary (rCHO) DG44 cells were examined. The supplementation of ZnSO4·7H2O up to 120 μM gradually increased specific mAb production rate of rCHO DG44 cells in the early growth phase (0-4 days of culture). The ZnSO4·7H2O concentration for enhancing mAb production without any cytotoxic effects on cell growth was 30-60 μM. In addition of 60 μM ZnSO4·7H2O to in-house protein-free medium and in-house chemically defined medium, mAb production was increased 2.0-fold and 6.5-fold, respectively. Moreover, addition of ZnSO4·7H2O to three kinds of commercial chemically defined media yielded a greater than 1.2-fold enhancement of mAb production. These data indicate that simple supplementation of a relatively high zinc ion concentration to cell culture media without significant changes of rCHO DG44 cell culture process can be useful for achieving high production of mAb.
研究了在重组中国仓鼠卵巢（rCHO）DG44细胞的化学成分确定的悬浮培养物中，添加高浓度的七水硫酸锌（ZnSO4·7H2O）对细胞生长和单克隆抗体（mAb）产生的影响。在培养早期（培养0-4天），添加高达120μM的ZnSO4·7H2O可逐渐提高rCHO DG44细胞的特异性mAb产生率。在对细胞生长无任何细胞毒性作用的情况下，提高mAb产生的ZnSO4·7H2O浓度为30-60μM。在自制的无蛋白培养基和自制的化学成分确定的培养基中添加60μM ZnSO4·7H2O后，mAb产量分别提高了2.0倍和6.5倍。此外，在三种商业化学成分确定的培养基中添加ZnSO4·7H2O，mAb产量提高了1.2倍以上。这些数据表明，在不显著改变rCHO DG44细胞培养过程的情况下，简单地向细胞培养基中添加相对高浓度的锌离子，对于实现mAb的高产可能是有用的。
