红色和远红色荧光探针的绝对双光子激发光谱。

Absolute two-photon excitation spectra of red and far-red fluorescent probes.

作者信息

Velasco Mary Grace M, Allgeyer Edward S, Yuan Peng, Grutzendler Jaime, Bewersdorf Joerg

出版信息

Opt Lett. 2015 Nov 1;40(21):4915-8. doi: 10.1364/OL.40.004915.

DOI:10.1364/OL.40.004915

Abstract

Efficient use of two-photon excitation (TPE) microscopy requires knowledge of the absolute TPE action cross sections (ATACSs) of fluorescent probes. However, these values are not available for recently developed dyes, which exhibit superior properties in many modern microscopy applications. We report ATACSs of five red to far-red organic dyes, ATTO 647N, STAR 635P, silicon rhodamine, ATTO 594, and ATTO 590. The dyes were found to have large ATACSs (>100 GM) at their respective wavelength peaks, thus supporting their use as bright fluorescent markers in TPE microscopy.

摘要

高效使用双光子激发（TPE）显微镜需要了解荧光探针的绝对双光子激发作用截面（ATACSs）。然而，对于最近开发的染料，这些值是不可用的，这些染料在许多现代显微镜应用中表现出优异的性能。我们报告了五种红色到远红色有机染料ATTO 647N、STAR 635P、硅罗丹明、ATTO 594和ATTO 590的ATACSs。发现这些染料在各自的波长峰值处具有较大的ATACSs（>100 GM），因此支持它们在TPE显微镜中用作明亮的荧光标记物。