Xu Chris, Zipfel Warren R
Cold Spring Harb Protoc. 2015 Mar 2;2015(3):250-8. doi: 10.1101/pdb.top086116.
This introduction reviews the multiphoton excitation cross sections of extrinsic and intrinsic fluorophores, genetically engineered probes, and nanoparticles. We will review the known two-photon excitation cross sections of biological indicators and will discuss several related issues such as how to theoretically estimate and experimentally gauge the two-photon cross section of an indicator. We provide practical guides for experimentally estimating the excitation cross section.
本引言回顾了外源性和内源性荧光团、基因工程探针以及纳米颗粒的多光子激发截面。我们将回顾生物指示剂已知的双光子激发截面，并讨论几个相关问题，例如如何从理论上估计以及通过实验测量指示剂的双光子截面。我们提供了实验估计激发截面的实用指南。
