Eskicioğlu Fatma, Özdemir Alper Tunga, Özdemir Rabia Bilge, Turan Gülüzar Arzu, Akan Zafer, Hasdemir Solmaz Pınar
a Department of Obstetrics and Gynecology , School of Medicine, Celal Bayar University , Manisa , Turkey .
b Department of Stem Cell , Institute of Health Sciences, Ege University , Izmir , Turkey .
J Matern Fetal Neonatal Med. 2016 Sep;29(18):3056-60. doi: 10.3109/14767058.2015.1114085. Epub 2015 Nov 30.
To determine role of human leukocyte antigen (HLA)-G, CD8, CD16, CD56, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α for recurrent miscarriages in feto-maternal interface.
Chorion and decidua samples were obtained from 11 women with unwanted pregnancies (healthy pregnancy, HP) and 10 women with missed abortion diagnosis after at least two pregnancy losses (recurrent miscarriage, RM). In addition, endometrial tissues were obtained from 10 non-pregnant women (NonP). The expressions of markers were evaluated using the Western blot analysis. The values obtained between different groups were compared.
The highest protein expression of CD56 was found in the HP compared to NonP and RM. Meanwhile, the lowest protein expression of CD16 was observed in the NonP compared to HP and RM. The HLA-G expression exhibited the highest level in HP; however, there was no statistically significant difference between groups. CD8 and IFNγ expressions were lowest in the NonP group; however, TNF-α was highest in the RM group.
The CD56 expression of uterine NK cells may be an indicator of a HP. However, not statistically significant, the increased expression of CD16, CD8, and also significantly increased expression of TNF may be associated with the predominant cytotoxic activity in the maternal immune system in patients with RM. Although there was no change in the expression of HLA-G, this finding may mean that the maternal immune system is unresponsive to HLA-G-mediated immunosuppressive signals originating from the fetus in these cases.
确定人类白细胞抗原（HLA）-G、CD8、CD16、CD56、干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α在母胎界面复发性流产中的作用。
从11例意外怀孕妇女（健康妊娠，HP）和10例至少经历两次妊娠丢失后诊断为稽留流产的妇女（复发性流产，RM）中获取绒毛和蜕膜样本。此外，从10例非妊娠妇女（NonP）中获取子宫内膜组织。使用蛋白质印迹分析评估标志物的表达。比较不同组之间获得的值。
与NonP和RM相比，HP中CD56的蛋白表达最高。同时，与HP和RM相比，NonP中CD16的蛋白表达最低。HLA-G表达在HP中表现出最高水平；然而，各组之间无统计学显著差异。NonP组中CD8和IFNγ表达最低；然而，RM组中TNF-α最高。
子宫自然杀伤细胞的CD56表达可能是健康妊娠的一个指标。然而，虽然无统计学显著差异，但CD16、CD8表达的增加以及TNF表达的显著增加可能与RM患者母体免疫系统中占主导地位的细胞毒性活性有关。虽然HLA-G的表达没有变化，但这一发现可能意味着在这些情况下，母体免疫系统对源自胎儿的HLA-G介导的免疫抑制信号无反应。
