Anderson Nicholas S, Kramer Eric A, Cezo James D, Ferguson Virginia L, Rentschler Mark E
J Biomech Eng. 2015 Dec;137(12):121010. doi: 10.1115/1.4031891.
Surgical tissue fusion devices ligate blood vessels using thermal energy and coaptation pressure, while the molecular mechanisms underlying tissue fusion remain unclear. This study characterizes the influence of apposition force during fusion on bond strength, tissue temperature, and seal morphology. Porcine splenic arteries were thermally fused at varying apposition forces (10-500 N). Maximum bond strengths were attained at 40 N of apposition force. Bonds formed between 10 and 50 N contained laminated medial layers; those formed above 50 N contained only adventitia. These findings suggest that commercial fusion devices operate at greater than optimal apposition forces, and that constituents of the tunica media may alter the adhesive mechanics of the fusion mechanism.
外科组织融合装置利用热能和贴合压力结扎血管，而组织融合背后的分子机制仍不清楚。本研究描述了融合过程中贴合力对粘结强度、组织温度和密封形态的影响。在不同的贴合力（10 - 500 N）下对猪脾动脉进行热融合。在40 N的贴合力下获得最大粘结强度。在10至50 N之间形成的粘结包含分层的中层；在50 N以上形成的粘结仅包含外膜。这些发现表明，商业融合装置在大于最佳贴合力的情况下运行，并且中膜成分可能会改变融合机制的粘附力学。
