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Proteolytic enzymes in excretory-secretory products from adult Nematospiroides dubius.

作者信息

Monroy F G, Cayzer C J, Adams J H, Dobson C

出版信息

Int J Parasitol. 1989 Feb;19(1):129-31. doi: 10.1016/0020-7519(89)90031-3.

DOI:10.1016/0020-7519(89)90031-3
PMID:2651341
Abstract

Excretory-secretory products (ES), collected from in vitro cultures of adult Nematospiroides dubius, were examined for proteolytic enzyme activity. ES enzymes had a pH optimum of 8.0 and their activity was sensitive to serine-proteinase inhibitors. Three SDS-resistant proteases were identified in ES at molecular weight (mol. wt) 200,000, 105,000 and 48,000 by incorporating substrates into the matrices of sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) gels.

摘要

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