Istituto Italiano di Tecnologia, IIT@CRIB, Largo Barsanti e Matteucci 53, 80125, Napoli, Italy.
Centro di Ricerca Interdipartimentale sui Biomateriali CRIB, Università di Napoli Federico II, Piazzale Tecchio 80, 80125, Napoli, Italy.
Adv Healthc Mater. 2015 Dec 9;4(17):2688-98. doi: 10.1002/adhm.201500588. Epub 2015 Oct 29.
The application of multimodal systems in the field of nanomedicine is advantageous as they can perform two or more tasks simultaneously. Here a robust approach is presented mimicking biogenic silica to design a multilayered nanocarrier system with a central oil core encapsulated within a polymer-silica shell. The outermost silica shell has been deposited through a biosilicification process induced by poly-L-lysine molecules immobilized on the surface of emulsion droplets. This system can be simultaneously loaded with high amount of hydrophobic molecules or contrasting agents in the inner oil core, while the polymeric-silica layers can be easily tagged with at least two different contrasting agents. Additionally, the zwitterionic nature of the silica precipitating peptide (poly-L-lysine) has been efficiently exploited to modulate and entirely reverse the surface charge of the nanocarrier without using any additional coating material. It has been demonstrated experimentally that the designed nanocapsular system is monodisperse, nontoxic, cargo protective, tunable in thickness, fluorescent, and magnetic resonance imaging (MRI) active so highly versatile for multiple applications in the field of drug delivery and in vivo imaging.
多模态系统在纳米医学领域的应用具有优势，因为它们可以同时执行两个或多个任务。在这里，我们提出了一种稳健的方法，模拟生物硅来设计一种具有中央油核的多层纳米载体系统，该油核被聚合物-硅壳包裹。最外层的硅壳是通过固定在乳液滴表面的聚-L-赖氨酸分子诱导的生物硅化过程沉积的。该系统可以同时在内油核中装载大量疏水分子或对比剂，而聚合物-硅层可以很容易地用至少两种不同的对比剂标记。此外，沉淀硅的肽（聚-L-赖氨酸）的两性离子性质被有效地利用来调节和完全反转纳米载体的表面电荷，而无需使用任何额外的涂层材料。实验证明，所设计的纳米胶囊系统具有单分散性、非毒性、货物保护性、可调厚度、荧光性和磁共振成像（MRI）活性，因此非常适合药物输送和体内成像领域的多种应用。
