Gedye Craig, Cardwell Tracy, Dimopoulos Nektaria, Tan Bee Shin, Jackson Heather, Svobodová Suzanne, Anaka Matthew, Behren Andreas, Maher Christopher, Hofmann Oliver, Hide Winston, Caballero Otavia, Davis Ian D, Cebon Jonathan
Ludwig Institute for Cancer Research, Austin Hospital, Studley Road, Heidelberg, VIC, 3084, Australia.
University of Newcastle, School of Biomedical Sciences and Pharmacy, Hunter Medical Research Institute, New Lambton Heights, NSW, 2305, Australia.
Stem Cell Rev Rep. 2016 Feb;12(1):156-61. doi: 10.1007/s12015-015-9630-8.
Cancer cell lines can be useful to model cancer stem cells. Infection with Mycoplasma species is an insidious problem in mammalian cell culture. While investigating stem-like properties in early passage melanoma cell lines, we noted poorly reproducible results from an aliquot of a cell line that was later found to be infected with Mycoplasma hyorhinis. Deliberate infection of other early passage melanoma cell lines aliquots induced variable and unpredictable effects on expression of putative cancer stem cell markers, clonogenicity, proliferation and global gene expression. Cell lines established in stem cell media (SCM) were equally susceptible. Mycoplasma status is rarely reported in publications using cultured cells to study the cancer stem cell hypothesis. Our work highlights the importance of surveillance for Mycoplasma infection while using any cultured cells to interrogate tumor heterogeneity.
癌细胞系对于模拟癌症干细胞可能是有用的。支原体属感染是哺乳动物细胞培养中一个潜伏性问题。在研究早期传代黑色素瘤细胞系的干细胞样特性时，我们注意到来自一个细胞系的一份样本结果重复性很差，该样本后来被发现感染了猪鼻支原体。故意感染其他早期传代黑色素瘤细胞系的样本，对假定的癌症干细胞标志物的表达、克隆形成能力、增殖和整体基因表达产生了可变且不可预测的影响。在干细胞培养基（SCM）中建立的细胞系同样易感。在使用培养细胞研究癌症干细胞假说的出版物中，支原体感染状态很少被报告。我们的工作强调了在使用任何培养细胞研究肿瘤异质性时监测支原体感染的重要性。
