印度西北部旁遮普邦MCP - 1基因 -2518 A/G多态性与乳腺癌的关联

Association of the -2518 A/G Polymorphism of MCP-1 with Breast Cancer in Punjab, North-West India.

作者信息

Sambyal Vasudha, Guleria Kamlesh, Kapahi Ruhi, Manjari Mridu, Sudan Meena, Uppal Manjit Singh, Singh Neeti Rajan

机构信息

Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India E-mail :

出版信息

Asian Pac J Cancer Prev. 2015;16(16):7243-8. doi: 10.7314/apjcp.2015.16.16.7243.

DOI:10.7314/apjcp.2015.16.16.7243

PMID:26514518

Abstract

BACKGROUND

Monocyte chemoattractant protein-1 (MCP-1) is a major chemokine thought to be responsible for monocyte and T-lymphocyte recruitment in acute inflammatory conditions and recruitment of macrophages in tumors. It is also implicated in cardiovascular disease, rheumatoid arthritis and chronic obstructive pulmonary disease. The aim of the present study was to investigate the correlation between MCP-1 -2518 A/G polymorphism and breast cancer risk in patients from Amritsar city of Punjab state in North-West India.

MATERIALS AND METHODS

We screened DNA samples of 200 sporadic breast cancer patients and 200 age and gender matched unrelated healthy individuals for MCP-1 -2518 A/G polymorphism using the PCR-RFLP method.

RESULTS

A significantly increased frequency of the GG genotype was observed in patients as compared to controls. Individuals carrying the MCP1 -2518GG genotype had a two fold risk for breast cancer (OR=2.06, 95%CI, 1.06-3.98; p=0.03). Genetic models analysis revealed a significant association between MCP-1 -2518 A/G polymorphism and cancer risk in homozygous co-dominant (OR=2.06, 95%CI, 1.06-3.98; p=0.03) and recessive (OR=1.97, 95%CI, 1.05-3.70; p=0.03) models.

CONCLUSIONS

We conclude that the GG genotype of the MCP-1-2518 A/G polymorphism is associated with increased risk to breast cancer in Punjab, North-West India.

摘要

背景

单核细胞趋化蛋白-1（MCP-1）是一种主要的趋化因子，被认为在急性炎症状态下负责单核细胞和T淋巴细胞的募集以及肿瘤中巨噬细胞的募集。它还与心血管疾病、类风湿性关节炎和慢性阻塞性肺疾病有关。本研究的目的是调查印度西北部旁遮普邦阿姆利则市患者中MCP-1 -2518 A/G多态性与乳腺癌风险之间的相关性。

材料与方法

我们使用PCR-RFLP方法对200例散发性乳腺癌患者以及200名年龄和性别相匹配的无关健康个体的DNA样本进行MCP-1 -2518 A/G多态性筛查。

结果

与对照组相比，患者中GG基因型的频率显著增加。携带MCP1 -2518GG基因型的个体患乳腺癌的风险增加两倍（OR=2.06，95%CI，1.06 - 3.98；p=0.03）。遗传模型分析显示，在纯合共显性（OR=2.06，95%CI，1.06 - 3.98；p=0.03）和隐性（OR=1.97，95%CI，1.05 - 3.70；p=0.03）模型中，MCP-1 -2518 A/G多态性与癌症风险之间存在显著关联。