通过反相高效液相色谱法对生物合成人胰岛素进行生产规模的纯化。

Production scale purification of biosynthetic human insulin by reversed-phase high-performance liquid chromatography.

作者信息

Kroeff E P, Owens R A, Campbell E L, Johnson R D, Marks H I

机构信息

Department of Biosynthetic Isolation and Purification Development, Lilly Research Laboratories, Indianapolis, IN 46285.

出版信息

J Chromatogr. 1989 Jan 6;461:45-61. doi: 10.1016/s0021-9673(00)94274-2.

DOI:10.1016/s0021-9673(00)94274-2

PMID:2651467

Abstract

A process based on reversed-phase high-performance liquid chromatography (RP-HPLC) has been developed for the purification of biosynthetic human insulin (BHI). The RP-HPLC procedure has been successfully integrated into the multimodal chromatographic production process used to purify kilogram quantities of BHI. Axial compression column technology was used in the scale-up process. The RP-HPLC procedure yields an insulin product having high chemical purity and full biological activity.

摘要

已开发出一种基于反相高效液相色谱（RP-HPLC）的方法来纯化生物合成人胰岛素（BHI）。RP-HPLC程序已成功整合到用于纯化数千克BHI的多模式色谱生产过程中。在放大过程中使用了轴向压缩柱技术。RP-HPLC程序产生的胰岛素产品具有高化学纯度和完全的生物活性。