Chen Ran, Deng Liang, Yu Xinxin, Wang Xiuxiu, Zhu Liye, Yu Tao, Zhang Yiheng, Zhou Bo, Xu Wentao, Chen Liang, Luo Haoshu
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Department of Animal Genetics and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, PR China.
Toxicol In Vitro. 2015 Dec 25;30(1 Pt B):264-73. doi: 10.1016/j.tiv.2015.10.011. Epub 2015 Oct 26.
Ochratoxin A (OTA) is a mycotoxin which has been shown to be nephrotoxic, hepatotoxic, and immunotoxic to animals, and mainly exists in the mildew grain. MicroRNAs (miRNAs) regulate a wide variety of cellular processes. However, the toxic effects of OTA on the germ cell and whether miRNAs mediate the effects of OTA-induced GC-2 cell apoptosis are still not clear. In the present study, OTA treatment resulted in a dose-dependent increase apoptosis in GC-2 cells. MiR-122 was increased in the OTA-treated GC-2 cells. It showed that Bcl-w was down-regulated after OTA treatment, and caspase-3 was obviously activated. Cyclin G1 (CCNG1) was significantly decreased, and inversely the expression of p53 was increased. Inhibition of miR-122 partly relieved the OTA-induced GC-2 cell apoptosis. These results indicate that OTA induces GC-2 cell apoptosis by causing the increase of caspase-3 activity and that miR-122 partly mediates the OTA-induced cell apoptosis.
赭曲霉毒素A（OTA）是一种霉菌毒素，已被证明对动物具有肾毒性、肝毒性和免疫毒性，主要存在于霉变谷物中。微小RNA（miRNA）调节多种细胞过程。然而，OTA对生殖细胞的毒性作用以及miRNA是否介导OTA诱导的GC-2细胞凋亡仍不清楚。在本研究中，OTA处理导致GC-2细胞凋亡呈剂量依赖性增加。OTA处理的GC-2细胞中miR-122增加。结果显示，OTA处理后Bcl-w下调，半胱天冬酶-3明显激活。细胞周期蛋白G1（CCNG1）显著降低，相反，p53的表达增加。抑制miR-122可部分缓解OTA诱导的GC-2细胞凋亡。这些结果表明，OTA通过导致半胱天冬酶-3活性增加诱导GC-2细胞凋亡，且miR-122部分介导OTA诱导的细胞凋亡。
