Birdsall Holly H, Hammond Timothy G
Departments of Otorhinolaryngology, Immunology, and Psychiatry, Baylor College of Medicine, Houston, TX 77030, USA.
Otolaryngology Section, Surgery Service Line, Durham VA Health Care System, 508 Fulton Street, Durham, NC 27705, USA.
J Toxicol. 2021 Apr 21;2021:6643324. doi: 10.1155/2021/6643324. eCollection 2021.
Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow. Diverse techniques to reintroduce shear stress have been studied in a variety of proximal tubular like cell culture models. These studies often have limited replicates because of the huge cost of equipment and do not report all relevant parameters to allow reproduction and comparison of studies between labs. This review codifies the techniques used to reintroduce shear stress, the cell lines utilized, and the biological outcomes reported. Further, we propose a set of interventions to enhance future cell biology understanding of nephrotoxicity using cell culture models.
药物性肾毒性导致巨大的发病率和死亡率，并造成巨大的经济成本。药物性急性肾损伤的最大负担落在近端肾小管细胞上。为维持其结构和功能，肾近端肾小管细胞需要来自肾小管液流的剪切应力。在各种近端肾小管样细胞培养模型中，人们研究了多种重新引入剪切应力的技术。由于设备成本高昂，这些研究的重复次数往往有限，而且没有报告所有相关参数，以便实验室之间进行研究的再现和比较。本综述整理了用于重新引入剪切应力的技术、所使用的细胞系以及所报告的生物学结果。此外，我们提出了一系列干预措施，以利用细胞培养模型增强未来细胞生物学对肾毒性的理解。
